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題名 | Effect of the HBV Whole-X Gene on the Expression of Hepatocellular Carcinoma Associated Proteins= |
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作者 | Zhang, Yu; Liu, Hongli; Cui, Meiling; Liu, Jinfeng; Yi, Ruitian; Niu, Yinghua; Chen, Tianyan; Zhao, Yingren; |
期刊 | Journal of Microbiology, Immunology and Infection |
出版日期 | 20160600 |
卷期 | 49:3 2016.06[民105.06] |
頁次 | 頁335-343 |
分類號 | 415.5332 |
語文 | eng |
關鍵詞 | HBV whole-X gene; Hepatitis B virus; Hepatocellular carcinoma; |
英文摘要 | Background: The hepatitis B virus (HBV) pre-X gene resides upstream of the HBV X gene, and together they form the HBV whole-X gene. Although it has been evident that the HBV whole-X protein is involved in the development of hepatocellular carcinoma, its biological role and molecular mechanism remain largely unknown. Methods: In this study, we subcloned the HBV whole-X gene and constructed a HBV whole-X expressing vector. After transfection of the HBV whole-X gene into HL-7702 cells, the profile of the differential cellular protein composition in the cells was analyzed by using twodimensional electrophoresis coupled to matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Results: The results showed that 18 major proteins were differentially expressed in the cells transfected with or without the HBV whole-X gene. The expression of these genes was further confirmed by reverse transcription-polymerase chain reaction and Western blot analysis. Conclusion: Our findings provide a new insight into the investigation of the pathological role that the HBV whole-X gene plays in the development of hepatocellular carcinoma and may lead to the design of novel strategies for the treatment of this disease. |
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