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題名 | The Antioxidant and Cytoprotective Activity of Ocimum Gratissimum Extracts against Hydrogen Peroxide-Induced Toxicity in Human HepG2 Cells= |
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作者 | Chiu, Yung-wei; Lo, Hung-jen; Huang, Hsin-yu; Chao, Pei-yu; Hwang, Jin-ming; Huang, Pei-yun; Huang, Shyh-jer; Liu, Jer-yuh; Lai, Te-jen; |
期刊 | Journal of Food and Drug Analysis |
出版日期 | 20130900 |
卷期 | 21:3 2013.09[民102.09] |
頁次 | 頁253-260 |
分類號 | 414.51 |
語文 | eng |
關鍵詞 | Antioxidants; Free radical scavenging activity; |
英文摘要 | Ocimum gratissimum is used as a traditional folk medicine in many countries. The objective of this study was to evaluate the antioxidant activities of an aqueous O. ratissimum extract (OGE) and to evaluate its cytoprotective activity against hydrogen peroxide-induced toxicity in human HepG2 cells. The results revealed that the total henolic content of the OGE reached 20%. In the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the OGE reduced up to 80% of the free radicals at a plateau concentration of 66.7 mg/mL. This indicates that OGE contains considerable free radical scavenging activity. In a dose-dependent manner, OGE pretreatment counteracted the decrease in cell viability in H2O2-treated HepG2 cells ( p < 0.05), and at a concentration of 20e80 mg/mL, it effectively reduced thiobarbituric acid reactive substance (TBARS) formation. These findings indicated that the aqueous extract of O. gratissimum has an antioxidant capacity and protective effect on oxidative stress in HepG2 cells. This makes OGE a promising therapeutic agent in liver diseases. |
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