頁籤選單縮合
題名 | Pyrosequencing Reveals an Oseltamivir-resistant Marker in the Quasispecies of Avian Influenza A (H7N9) Virus |
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作者姓名(外文) | Mok, Chee-keng; Chang, Shih-cheng; Chen, Guang-wu; Lo, Yu-lun; Chen, Shu-jen; Wu, Ho-sheng; Liu, Ming-tsan; Chang, Feng-yee; Lin, Tzou-yien; Shih, Shin-ru; | 書刊名 | Journal of Microbiology, Immunology and Infection |
卷期 | 48:4 2015.08[民104.08] |
頁次 | 頁465-469 |
關鍵詞 | Avian influenza A virus; H7N9; Oseltamivir-resistant marker; Pyrosequencing; |
語文 | 英文(English) |
英文摘要 | Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan’s first imported human case and found R and K in quasispecies. |
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