查詢結果分析
來源資料
相關文獻
- 利用聚合酶連鎖反應-變性梯度膠體電泳方法分析診斷家貓體染色體顯性多囊性腎臟病之初步探討
- The Association between Single-Nucleotide Polymorphisms in the Inosine Triphosphate Pyrophosphatase Gene and Ribavirin-induced Hemolytic Anemia in Genotype 2 Hepatitis C Virus-infected Taiwanese Patients Receiving Pegylated Interferon and Ribavirin Combination Therapy
- 臺灣稉型水稻品種隨機型SNP分子標誌資料庫的建構
- 利用多重單一核苷酸多型性鑑定水稻品種純度
- Dynamic Programming for Single Nucleotide Polymorphism ID Identification in Systematic Association Studies
- Tumor Necrosis Factor-[8e60]Gene Promoter Polymorphism in Chinese Patients with Systemic Lupus Erythematosus
- 西瓜雜交種子純度檢測用SNP標誌之建立
- 稻熱病抗性相關分子標誌之開發與臺灣水稻品種Pik基因座單倍型分析
- 有效計算智慧為主的方法用於誘發突變引子設計
- 利用多重單一核苷酸基因定型技術鑑別國、內外食米品種
頁籤選單縮合
題名 | 利用聚合酶連鎖反應-變性梯度膠體電泳方法分析診斷家貓體染色體顯性多囊性腎臟病之初步探討=A Preliminary Study of Using the PCR-DGGE Assay for Detection the Autosomal Dominant Polycystic Kidney Disease in Domestic Cats |
---|---|
作者姓名(中文) | 謝玉貞; 鍾楊聰; 曾秋隆; | 書刊名 | 農林學報 |
卷期 | 60:3 2011.09[民100.09] |
頁次 | 頁199-207 |
分類號 | 437.25 |
關鍵詞 | 貓之體染色體顯性多囊性腎臟病; 聚合酶連鎖反應-變性梯度膠體電泳方法; 腎臟超音波造影; 單一核苷酸多型性; Autosomal dominant polycystic kidney disease; Polymerase chain reaction-denaturing gradient gel electrophoresis; Renal ultrasound; Single nucleotide polymorphisms; |
語文 | 中文(Chinese) |
中文摘要 | 貓之體染色體顯性多囊性腎臟病( ADPKD)被認為是貓最常見家族性遺傳性疾病,常發生在波絲貓或是有波絲貓血統的貓。貓 PKD1等位基因第 29外顯子( exon)中的第 3284個核苷酸轉換( C→A),所導致之點突變,是造成 AD-PKD原因之一。目前臨床上主要使用腎臟超音波造影檢測方式來診斷 ADPKD,本研究首先從 2009至 2010年間至中興大學獸醫教學醫院就診的貓隻,共收集 7個樣本,利用腎臟超音波造影檢測初步診斷得到 AD-PKD之 4樣本,進而利用 PCR-DGGE檢測,結果亦可檢測出 4個樣本有 PKD1等位基因外顯子突變。由此研究結果顯示利用 PCRDGGE鑑別 AD-PKD突變株的靈敏度及準確度與腎臟超音波造影檢查結果一致,故在診斷 AD-PKD上提供另外一種分子鑑定分析技術之選擇。 |
英文摘要 | The autosomal dominant polycystic kidney disease (AD-PKD) is a familial disease in cats and is characterized by the occurrence of fluid-filled cysts in the renal tissues. The cysts often lead to the kidney failure. The major cause of disease is a C→A point mutation at nt 3284 in the PKD1 gene. In clinical diagnosis, the renal ultrasounds are often used to diagnose AD-PKD. In this study, 7 blood samples were collected and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method to detect single nucleotide polymorphisms (SNPs) in PKD1 gene. Among the 7 samples, 4 samples were preliminarily diagnosed with AD-PKD by renal ultrasounds. The 7 samples were further analyzed by PCRDGGE, and only 4 samples were diagnosed with AD-PKD. Therefore, the sensitivity and the accuracy of PCR-DGGE provides an alternative technique to distinguish AD-PKD mutants in cats. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。