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題 名 | Differentiation of Blood T Cells: Reprogramming Human Induced Pluripotent Stem Cells into Neuronal Cells |
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作 者 | Tsai, Ping-hsing; Chang, Yun-ching; Lee, Yi-yen; Ko, Yu-ling; Yang, Yu-hsuan; Lin, Chun-fu; Chang, Yuh-lih; Yu, Wen-chung; Shih, Yang-hsin; Chen, Ming-teh; | 書刊名 | Journal of the Chinese Medical Association |
卷 期 | 78:6 2015.06[民104.06] |
頁 次 | 頁353-359 |
分類號 | 368.5 |
關鍵詞 | Electroporation; Epstein-Barr nuclear antigen-1; Human-induced pluripotent stem cells; Nonviral induced pluripotent stem cells; T cells; |
語 文 | 英文(English) |
英文摘要 | Background Human induced pluripotent stem cells (iPSCs) morphologically and functionally resemble human embryonic stem cells, which presents the opportunity to use patient-specific somatic cells for disease modeling and drug screening. In order to take one step closer to clinical applications, it is important to generate iPSCs through a less invasive approach and from any accessible tissue, including peripheral blood. Meanwhile, how to differentiate blood cell-derived iPSCs into neuron-like cells is still unclear. Methods We utilized Epstein–Barr nuclear antigen-1-based episomal vectors, a nonviral system that can reprogram somatic cells into iPSCs in both feeder-dependent and feeder-free conditions, to generate iPSCs from T cells via electroporation and then induce them into neuronal cells. Results We successfully isolated sufficient T cells from 20 mL peripheral blood of the donors and reprogrammed these T cells into iPSCs within 4 weeks. These iPSCs could be stably passaged to at least 50 passages, and exhibited the abilities of pluripotency and multiple-lineage differentiation. Notably, under the medium induction for 21 days, these T-cell-derived iPSCs could be differentiated into Nestin (neural progenitor marker)-, GFAP (glial cell marker)-, and MAP2 (neuron cell marker)-positive cells detected by immunofluorescence methods. Conclusion We have developed a safer method to generate integration-free and nonviral human iPSCs from adult somatic cells. This induction method will be useful for the derivation of human integration-free iPSCs and will also be applicable to the generation of iPSCs-derived neuronal cells for drug screening or therapeutics in the near future. |
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