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題名 | 合成具過氧化酶活性之銀奈米粒子/多壁奈米碳管奈米複合材料應用於過氧化氫和葡萄糖偵測=Synthesis of the Peroxidase-like Composites with Silver Nanoparticles/Multi-walled Carbon Nanotubes Nanomaterial and Its Applications for Detecting Hydrogen Peroxide and Glucose |
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作 者 | 莊宇晨; 邱松權; 邱泰嘉; 胡焯淳; | 書刊名 | 化學 |
卷期 | 73:1 2015.03[民104.03] |
頁次 | 頁3-11 |
專輯 | 生物分析化學 |
分類號 | 341 |
關鍵詞 | 多壁奈米碳管; 銀奈米粒子; 奈米複合材料; 仿過氧化酶活性; Multi-walled carbon nanotubes; Silver nanoparticles; Nanocomposites; Peroxidase-like activity; |
語文 | 中文(Chinese) |
中文摘要 | 本研究使用銀奈米粒子 (Ag NPs) 與多壁奈米碳管 (multi-walled carbon nanotubes, MWCNTs)合成奈米複合材料 (Ag NPs-MWCNTs),並利用 TEM、FT-IR、XPS和 Zeta電位等儀器鑑定奈米複合材料的特徵,研究發現 Ag NPs-MWCNTs奈米複合材料具有仿酵素-過氧化酶 (peroxidase) 活性。從實驗結果證明與天然酵素辣根過氧化物酶 (horseradish peroxidase, HRP) 比較,Ag NPs-MWCNTs奈米複合材料展現出較佳的溫度及 pH值穩定性,即使在嚴苛的條件下,此奈米複合材料仍能展現其催化活性。本研究使用 Tris-borate 100 mM (pH 7.0) 作為緩衝溶液,開發出偵測過氧化氫與葡萄糖濃度的條件,過氧化氫偵測時濃度範圍 70 μM~600 μM,LOD = 52 μM,R2值為 0.9962;偵測葡萄糖濃度範圍為 0.1 mM~1 mM,LOD = 0.06 mM,R2值為 0.9942。此複合材料具有良好的催化活性,並且從選擇性實驗結果得知,此方法並不會受到其他醣類的干擾,未來可望應用於血清中葡萄糖的檢測。 |
英文摘要 | In this study, the nanomaterials were synthesized from the silver nanoparticles (Ag NPs) and modified multi-walled carbon nanotubes (MWCNTs). We found that the Ag NPs-MWCNTs nanomaterial have enzyme-peroxidase activity and could be used as molecular sensor. The mixture of the modified multi-walled carbon nanotubes (0.5 mg mL-1), silver ions (25 mM) and sodium hydroxide (8 M) was stand for 30 min at 90 °C to form the nanomaterials. We characterized the as-prepared nanomaterial by TEM, FT-IR, XPS, and Zeta potential. The results demonstrate that the Ag NPs-MWCNTs nanomaterial exhibit higher thermal and pH durance than the nature enzyme (HRP), and thus could be suitable for a wider range of harsh conditions. Our study developed a new method for the detection of hydrogen peroxide and glucose by using these nanomaterials as probes in 100 mMTris-borate (pH 7.0) buffer solution. The linear range of hydrogen peroxide was from 70 μM to 600 μM, LOD = 52 μM, R2 = 0.9962; the linear range of glucose was from 0.1 mM to 1 mM, LOD = 0.06 mM, R2 = 0.9942. This method has satisfied sensitivity and selectivity and can be further used to the detection of glucose in serum. |
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