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題 名 | Analysis of Humoral Immunity of Hepatitis D Virus DNA Vaccine Generated in Mice by Using Different Dosage, Gene Gun Immunization, and in Vivo Electroporation=分析D型肝炎DNA疫苗以不同劑量、基因槍免疫法及體內電穿孔法在小鼠中的體液免疫反應 |
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作 者 | 蕭奕宗; 黃怡翔; 吳肇卿; 陶秘華; 許萬枝; 張扶陽; 李壽東; | 書刊名 | 中華醫學會雜誌 |
卷 期 | 69:1 民95.01 |
頁 次 | 頁7-13+CA02 |
分類號 | 418.293 |
關鍵詞 | DNA疫苗; 基因槍; D型肝炎病毒; 體內電穿孔法; DNA vaccine; Gene gun; Hepatitis D virus; In vivo electroporation; |
語 文 | 英文(English) |
英文摘要 | Background: Hepatitis D virus (HDV) DNA vaccine can produce Th1 and cytotoxic T-cell immune responses but only a low anti-HDV antibody titer is generated with a large hepatitis D antigen (L-HDAg) construct. In contrast, DNA vaccine expressing small hepatitis D antigen (S-HDAg) can generate a high titer of anti-HDV antibodies. Whether the low humoral immunity of L-HDAg DNA vaccine is due to inadequate dosage or can be ameliorated by other modes of immunization needs further evaluation. Methods: Plasmid (p25L) encoding L-HDAg and plasmid (pS/p25L) coexpressing hepatitis B surface antigen (HBsAg) and L-HDAg were used in this study. We compared the humoral response generated in mice using different plasmid DNA dosages and modes of immunization, including gene gun and in vivo electroporation (EP). Results: Intramuscular injection with a high dose of plasmid DNA (10 mg/kg) produced strong antibodies to HBsAg earlier than the usual dose did, but did not augment the anti-HDV response. Gene gun DNA immunization could not provide a better humoral immune response to HDV. EP DNA immunization had a higher anti-HDV seroconversion rate of 80%, but the anti-HDV antibody responses were generally weak (titer ≤ 400:1). Conclusion: The low humoral immunogenicity of DNA vaccine with L-HDAg cannot be ameliorated by different dosage, gene gun immunization, or in vivo EP intramuscular injection. DNA vaccine with a L-HDAg construct may not be a candidate HDV vaccine to generate anti-HDV humoral immunity. |
本系統中英文摘要資訊取自各篇刊載內容。