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題 名 | 建立以即時聚合酶鏈反應技術偵測水禽小病毒=Detection of Waterfowl Parvovirus with Real-time Polymerase Chain Reaction |
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作 者 | 陳燕萍; 李敏旭; 李淑慧; | 書刊名 | 行政院農業委員會家畜衛生試驗所研究報告 |
卷 期 | 41 民95.12 |
頁 次 | 頁79-86 |
分類號 | 437.246 |
關鍵詞 | 即時聚合酶鏈反應; 水禽小病毒; 鵝小病毒; 正番鴨小病毒; Real-time polymerase chain reaction; Waterfowl parvovirus; Goose parvovirus; Muscovy duck parvovirus; |
語 文 | 中文(Chinese) |
中文摘要 | 為建立以即時聚合酶鏈反應偵測水禽小病毒,利用Applied Biosystems 7500Real-Time PCR System配合SYBR green方法進行水禽小病毒核酸的偵測,鑑於核酸分子方面分為鵝小病毒與正番鴨小病毒,故試驗中選擇病毒基因保留性較高區域,設計鵝小病毒與正番鴨小病毒專一性之引子,建立以即時聚合酶鏈反應技術偵測水禽小病毒之反應條件,至少可測得至3 pg之核酸濃度,較一般PCR敏感約10~100倍,並建立核酸與循環數之線性關係,可作為核酸標準曲線之參考,進而應用於核酸定量之研究。 |
英文摘要 | The purpose of the study was to develop the assay of detection of warerfowl parvovirus with real-time polymerase chain reaction by proceeding with the Applied Biosystems 7500 Real-Time PCR System coupled with SYBR green. Waterfowl parvovirus was subdivided into goose parvovirus and Muscovy duck parvovirus. According to the conserved region of GPV and MDPV respectively, the specific primers were designed for real-time PCR. The constructed real-time PCR assay has a detection limit of 3 pg. The sensitivity was 100-fold higher than common PCR. The developed linear relationship between concentration of nucleic acid and cycle numbers of reaction was could be the base of the standard curve of nucleic acid, and further, it could be applied for quantifying the nucleic acid. |
本系統中英文摘要資訊取自各篇刊載內容。