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題名 | 利用葉圓片接種法測定甜瓜白粉病菌Podosphaera xanthii對克收欣之感受性=Detection of Kresoxim-methyl Resistance of Podosphaera xanthii with Leaf-disc Method |
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作 者 | 黃晉興; | 書刊名 | 植物病理學會刊 |
卷期 | 22:4 2013.12[民102.12] |
頁次 | 頁353-366 |
分類號 | 435.261 |
關鍵詞 | 甜瓜白粉病菌; 克收欣; 抗藥性; Podosphaera xanthii; Kresoxim-methyl; Chemical resistance; |
語文 | 中文(Chinese) |
中文摘要 | 為了檢試甜瓜白粉病菌菌株對克收欣的抗感藥性,建立葉圓片接種測定法,其方法為:將甜瓜葉圓片置放於盛有不同濃度克收欣之培養皿內,再接種甜瓜白粉病菌Podosphaera xanthii,於24/20°C(日/夜)光照12小時的環境下培養10天,判讀發病度。結果顯示感藥性菌株在處理1或10 a. i. mg/L以上之有效濃度時,無法造成病害,而抗藥性菌株在1000 a. i. mg/L之處理仍可造成嚴重病害;供試菌株亦於盆栽植株上出現相同的抗感藥性反應。克收欣抗或感藥性的甜瓜白粉病菌菌株接種在供試17種甜瓜品種的葉圓片,抗感藥菌株在不同品種上均出現相同抗感藥性反應。處理克收欣100 a. i. mg/L可明顯抑制感藥性菌株之分生孢子在葉圓片上發芽及菌絲生長,對抗藥性菌株則否。抗克收欣之菌株亦對同屬於史托比系藥劑的亞托敏、百克敏及三氟敏有抗藥性,但對其他非史托比系藥劑如平克座與快諾芬則無抗藥性。2004-2010年從台南市分離得到5個甜瓜白粉病菌菌株Podosphaera xanthii皆對克收欣有抗藥性,然而從台中市霧峰區分離得到5個菌株,2010年以前分離得到3株菌株皆為感藥性,而於2010年分離得到的2株菌株皆為抗藥性。本文結果顯示抗克收欣的甜瓜白粉病菌已在檢測田區出現。 |
英文摘要 | Leaf-disc assay was established for detecting kresoxim-methyl susceptibility of Podosphaera xanthii, the causal agent of melon powdery mildew. From the susceptibility test of P. xanthii on leaf-discs, the susceptible isolates did not cause disease at 1 or 10 a. i. mg/L; whereas, the resistant ones even caused severe disease at 1000 a. i. mg/L. The susceptibility reactions of susceptible or resistant P. xanthii isolates against kresoxim-methyl on the potted plant test were similar with those on leaf-discs. Furthermore, the susceptibility reactions showed similar result on leaf-disc assay of 17 tested melon varieties. When the leaf-discs were treated with 100 a. i. mg/L of kresoxim-methyl, the conidia germination, hyphae development, and hyphal length of the susceptible isolates were inhibited, but not the resistant isolates. With the leaf-disc assay, those isolates which were resistant to kresoxim-methyl were also resistant to azoxyrstrobin, pyraclostrobin and trifloxystrobin, which belong to strobilurin chemicals, but susceptible to penconazole and quinoxyfen (not strobilurin chemicals). From 2004 to 2010, 5 isolates of P. xanthii were isolated from Tainan, and all were resistant to kresoxim-methyl. Other five isolates were isolated form Taichung, and only I isolate from 2010 was resistant to kresoxim-methyl. The results demonstrated the presence of kresoxim-methyl resistant isolates of P. xanthii in the test fields. |
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