頁籤選單縮合
題名 | 羥四環素對蜜蜂美洲幼蟲病的防治效果與蜂蜜中的殘留檢測=The Effect of Oxytetracycline to Control American Foulbrood in Honeybee, and the OTC Residue in Honey |
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作者 | 柯仲宇; 鄭浩均; 陳春廷; 陳裕文; | 書刊名 | 臺灣昆蟲 |
卷期 | 33:1 2013.03[民102.03] |
頁次 | 頁1-13 |
分類號 | 433.3 |
關鍵詞 | 美洲幼蟲病; 西洋蜂; 羥四環素; 防治; 殘留; American foulbrood; Honeybee; Oxytetracycline; Control; Residue; |
語文 | 中文(Chinese) |
中文摘要 | 美洲幼蟲病(American foulbrood, AFB)是一種危害西洋蜂(Ap^s mellifera L.)的重要細菌性病害,病原為可形成抱子的幼蟲芽抱桿菌(Paenibacillus laruae)。由諸多文獻報導顯示該病原已對常用的經四環素(oxytetracycline, OTC)產生抗性,本文利用對OTC具耐藥性的幼蟲芽孢桿菌台灣本土分離株做為接種源, 以探討OTC的防治藥效;並且探討以搖除儲蜜的方式,減低OTC殘留於蜂產品的風 險。在防治藥效評估方面,選取8群健康西洋蜂的1日齡幼蟲接種本土具耐藥性的 P. larvae孢子,並於試驗期間分別給予一次800 mL含有OTC 50 mg與125 mg 二 種劑量的糖水,結果顯示兩種處理皆能有效抑制AFB病徵的發生。在藥劑殘留量方 面,所有蜂群均於施藥後第10、20及30日分別搖去儲蜜,並檢測蜂蜜中OTC的殘 留量。結果顯示,在施藥後30日的儲蜜樣本中,施用50 mg OTC劑量的蜂群均未 檢測出殘留,而施用125 mg的蜂群則有2群有檢出殘留,分別為8 ppb及25 ppb。 綜合以上結果顯示,OTC對台灣本土耐藥性病原仍具良好的防治效果,但施藥蜂群 須經過2次以上的搖除儲蜜,才能將蜂蜜中羥四環素殘留量降低至25 ppb以下。 |
英文摘要 | American foulbrood (AFB) is a severe bacterial disease in larval honeybee (Apis mellifera) caused by the spores of Paenibacillus larvae. Because several reports have documented oxytetracycline (OTC) resistance in P. larvae in some regions, we used OTC-tolerated spores isolated from an apiary in Taiwan as the source of inoculum to conduct a field experiment to test the efficacy of OTC and determine the OTC residue levels in the honey extracted. The 1-day-old larvae from 8 strong honeybee colonies were each inoculated by P. larvae spores, and the colonies were fed sugar syrup containing 50 and 125 mg OTC respectively. The results showed that two doses of OTC could effectively control AFB. All honey of the experimental colonies was removed at 10, 20 and 30 days after medication to check for OTC residue in the honey. The results showed that there was no detectable residue left in the honey at 30 days after the 50 mg OTC medication, but 8 and 25 ppb were detected respectively in two of the colonies that had received a 125 mg dosage application on the same sampling date. Our results show that OTC medication still provides good control against infection of OTC-tolerated spores. However, the contaminated honey needs to be removed twice in a row to decrease the OTC residues in the honey to below the required 25 ppb. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。