頁籤選單縮合
題 名 | Effect of m-3M3FBS on Ca□ Movement in PC3 Human Prostate Cancer Cells |
---|---|
作 者 | Tsai, Jeng-yu; Shieh, Pochuen; Kuo, Daih-huang; Chen, Fu-an; Kuo, Chun-chi; Jan, Chung-ren; | 書刊名 | 中國生理學雜誌 |
卷 期 | 53:3 2010.06[民99.06] |
頁 次 | 頁151-159 |
分類號 | 415.863 |
關鍵詞 | Ca□; m-3M3FBS; PC3; Prostate; |
語 文 | 英文(English) |
英文摘要 | Abstract The effect of 2,4,6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benzenesulfonamide (m-3M3FBS), a presumed phospholipase C activator, on cytosolic free Ca2+ concentrations ([Ca2+]i) in PC3 human prostate cancer cells is unclear. This study explored whether m-3M3FBS changed basal [Ca2+]i levels in suspended PC3 cells by using fura-2 as a Ca2+-sensitive fluorescent dye. M-3M3FBS at concentrations between 10-50 μM increased [Ca2+]i in a concentration-dependent manner. The Ca2+ signal was reduced by 60% by removing extracellular Ca2+. M-3M3FBS-induced Ca2+ influx was inhibited by the storeoperated Ca2+ channel blockers nifedipine, econazole and SK&F96365, and by the phospholipase A2 inhibitor aristolochic acid. In Ca2+-free medium, 30 μM m-3M3FBS pretreatment greatly inhibited the [Ca2+]i rise induced by the endoplasmic reticulum Ca2+ pump inhibitor thapsigargin or BHQ. Conversely, pretreatment with thapsigargin, BHQ or cyclopiazonic acid reduced the major part of m-3M3FBSinduced [Ca2+]i rise. Inhibition of phospholipase C with U73122 did not much alter m-3M3FBS-induced [Ca2+]i rise. Collectively, in PC3 cells, m-3M3FBS induced [Ca2+]i rises by causing phospholipase Cindependent Ca2+ release from the endoplasmic reticulum and Ca2+ influx via store-operated Ca2+channels. |
本系統中英文摘要資訊取自各篇刊載內容。