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題 名 | 基於空間時域聚焦的廣視域多光子顯微術於快速光學切片上應用=Spatiotemporal Focusing-based Widefield Multiphoton Microscopy for Fast Optical Sectioning |
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作 者 | 鄭力中; 張家源; 陳顯禎; | 書刊名 | 光學工程 |
卷 期 | 118 2012.06[民101.06] |
頁 次 | 頁33-40 |
分類號 | 448.59 |
關鍵詞 | 空間時域聚焦; 廣視域多光子顯微術; 光學切片; 適應性光學; Spatiotemporal focusing; Widefield multiphoton microscopy; Optical sectioning; Adaptive optics; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究主要以空間與時域同時聚焦所發展出的廣視域多光子激發顯微術應用於快速光學切片上,目前這套顯微術可達100Hz以上的高速度取像,而激發面積為100x200μm^2,側向解析度小於0.5μm以及縱向解析度3.5μm。實驗中,利用此技術來觀察0.5μm大小的螢光球其布朗運動情形,而雞腱的二倍頻訊號也可在此套廣視域多光子顯微鏡下以100Hz的幀速度取得,提供快速且具有高縱向解析的生物影像。另外,為了補償時域面干擾對系統的影響,此顯微技術同時整合了影像式適應性光學系統,對固定在洋菜膠的1μm螢光球在不同深度的干擾修正有不錯效果。 |
英文摘要 | A microscope based on spatiotemporal focusing offering widefield multiphoton excitation has been developed to provide fast optical sectioning images. This configuration can produce multiphoton images with an excitation area larger than 200 x 100 μm^2 at a frame rate greater than 100 Hz. Brownian motions of fluorescent microbeads as small as 0.5μm were observed in real-time with a lateral spatial resolution of less than 0.5μm and an axial resolution of approximately 3.5μm. Also, second harmonic images of chicken tendons demonstrate that the developed widefield multiphoton microscope can provide high resolution z-sectioning for bioimaging. Furthermore, in order to compensate temporal profile distortions, a sensorless image-based adaptive optics system was integrated into the microscope. The two-photon excited fluorescence image quality of 1μm fluorescent beads sealed in agarose gel at different depths is improved. |
本系統中英文摘要資訊取自各篇刊載內容。