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題名 | 以生物配位體模式評估鉀和鈣對大豆鎘毒性的減緩效應=A Biotic Ligand Model (BLM) Used for Assessing Alleviation Effects of Potassium and Calcium on Cadmium Toxicity to Soybean |
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作者 | 何珮綺; 游淳卉; 陳柏青; 莊愷瑋; Ho, Pei-chi; Yu, Chun-hui; Chen, Bo-ching; Juang, Kai-wei; |
期刊 | 作物、環境與生物資訊 |
出版日期 | 20120300 |
卷期 | 9:1 2012.03[民101.03] |
頁次 | 頁1-14 |
分類號 | 434.218 |
語文 | chi |
關鍵詞 | 重金屬; 植物毒性; 水耕試驗; 劑量反應曲線; 競爭吸附; Heavy metal; Phytotoxicity; Hydroponic culture; Dose-response curve; Competitive adsorption; |
中文摘要 | 由於陽離子的競爭對重金屬的生物毒性和有效性扮演很重要的角色,生物配位體模式(biotic ligand model;BLM)因此被提出,以利用於評估大量元素陽離子(如:Ca(上標 2+)、Mg(上標 2+)、K(上標 +)、Na(上標 +)和H(上標 +))對重金屬生物毒性的減緩效應。BLM的基本假設是重金屬毒性來自金屬離子與生物結合位置的反應,大量元素可能競爭生物結合位置而促成重金屬毒性的降低。本研究目的,在以BLM探討鉀和鈣減緩大豆[Glycine max (L.)Merr.]鎘毒性的效應。本研究以短期(7 d)的大豆水耕試驗進行不同鎘濃度的曝露處理(0、4.5、8.9、13.3和17.8 μM),測量相對株高伸展量(relativeextension of plant height; REP)和相對根長(relative root length; RRL)以作為植株對鎘曝露處理的生長反應。分別以調整不同鉀(0.04、0.4、4 mM)和鈣(0.02、0.2、2 mM)濃度的溶液進行鎘曝露試驗,再求出降低植株REP 和RRL 生長反應50%的鎘曝露量(EC50(下標{Cd(上標 2+)})),以作為毒性指標,然後依試驗資料建構出評估大豆鎘毒性的BLM。結果顯示,大豆幼苗的REP和RRL隨鎘曝露量上升而下降,而且EC50{Cd2+}會隨增加試驗溶液中鉀和鈣濃度而呈直線上升的趨勢,此結果表示提升鉀和鈣濃度確實能減緩鎘對大豆幼苗的毒性。由本研究求出的BLM參數中,對應減緩RRL和REP的鎘毒性反應所求出之鉀穩定常數對數值(logK(下標 KBL))分別為2.51和2.57,而鈣穩定常數對數值(logK(下標 CaBL))則分別為2.62和2.85,表示鈣比鉀對生物配位體有較大的競爭能力,對減緩鎘毒性的效應也較強。再者,對應降低RRL和REP生長反應50%的鎘與生物配位體結合比例(f(上標 50)(下標 CdBL))分別為0.445和0.207,表示相對於RRL,REP對鎘毒性的反應較敏感,因此大豆幼苗植株地上部的鎘毒性反應較植株地下部更為明顯。 |
英文摘要 | As cation competition plays an important role in biological toxicity and availability of heavy metals, a biotic ligand model (BLM) has been proposed as a tool to quantitatively evaluate the alleviation effect of macro element cations (e.g., Ca(superscript 2+)), Mg(superscript 2+)), K(superscript +), Na(superscript +), and H(superscript +)) on phytotoxicity of heavy metals. The BLM is based on the heavy-metal toxicity caused by free metal ions reacting with biological binding sites. The macro cations might compete with metal ions for the binding sites and decrease the toxicity of metals. The purpose of the present study was to investigate the alleviation of cadmium (Cd) toxicity in soybean (Glycine max L. Merr.) induced by potassium (K) and calcium (Ca) with a BLM. A short term (7 d) experiment using soybean seedlings grown in a hydroponic culture with Cd concentrations ranged from 0 to 17.8 μM was carried out for the study. The relative extension of plant height (REP) and relative root length (RRL) of soybean seedlings were measured for investigating the growth responses to Cd exposures. The 50% effective activities of Cd resulting in 50% growth reduction (EC50(subscript {Cd(superscript 2+)})) for REP and RRL were used as the toxicity indices for two sets of Cd exposures in which the concentrations of Ca (0.02, 0.2, and 2 mM) and K (0.04, 0.4, and 4 mM) were varied, respectively. A BLM for assessing Cd toxicity to soybean was then constructed. Results showed that REP and RRL were decreased with increase of Cd exposures. The EC50(subscript {Cd(superscript 2+)})) values of RRL and REP were linearly increased with the increment of Ca and K concentrations, suggesting that increases of Ca and K concentrations could alleviate Cd toxicity to soybean seedlings. The BLM parameters, logarithmic binding stability constants of K (log K(subscript KBL)), obtained for RRL and REP were 2.51 and 2.57, respectively, while the constants of Ca (log K(subscript CaBL)) obtained for RRL and REP were 2.62 and 2.85, respectively. Results indicated that Ca competed for the biotic ligands more strongly than K did, and hence Ca treatment had better alleviation effect than that of K treatment. The fractions of the total biotic ligand sites bound by Cd (f(superscript 50)(subscript CdBL) ) for RRL and REP were 0.445 and 0.207, respectively, implying that REP was more sensitive than RRL upon Cd toxicity. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。