頁籤選單縮合
題名 | 野生狼尾草種原RAPD標誌之群叢分析=Cluster Analysis of Germplasms of Pennisetum purpureum with RAPD Markers |
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作者 | 林正斌; 李姿蓉; 林明村; 蘇建安; 侯金日; Lin, Jeng-bin; Lee, Tzu-rung; Lin, Ming-tsun; Sung, Cheng-zan; Hou, Chin-jin; |
期刊 | 中華民國雜草學會會刊 |
出版日期 | 20110600 |
卷期 | 32:1 2011.06[民100.06] |
頁次 | 頁23-33 |
分類號 | 434.4 |
語文 | chi |
關鍵詞 | 狼尾草; 種原; 逢機增殖多型性; 群叢分析; Pennisetum purpureum; Germplasm; RAPD; Cluster analysis; |
中文摘要 | 為探討臺灣野生狼尾草 (Pennisetum purpureum) 之分佈及其遺傳歧異度,本試驗自臺灣 15 個地區搜集 104 個樣本,並以狼尾草臺畜草 2 號為對照,利用逢機增殖多型性 DNA (random amplified polymorphic DNA, RAPD)技術,分析樣本間之遺傳歧異度。結果顯示,以 AMOVA (analysis of molecular variance)進行變方分析, 顯示族群內變方佔總變方之94.18% (p<0.001)且達顯著性差異。各縣市之遺傳距離介於 0.0003 至 0.3 之間,以遺傳距離 0.03 為截點則可將 104 個樣本分為 7 群,分別為第 1 群:臺北、新竹;第 2 群:苗栗;第 3 群:桃園;第 4 群:臺東、屏東;第 5 群:臺中、南投、花蓮、彰化、雲林;第 6 群:臺南、嘉義、高雄;第 7群:宜蘭。遺傳距離與樹狀圖的相關係數 r 為 0.68。綜合上述,此 7 群狼尾草種原足以進一步進行選拔評估,以期在短時間內篩選出具高品質潛力之狼尾草。 |
英文摘要 | Napiergrass (Pennisetum purpureum) is one of the most important forage crops in Taiwan. The hybrid seeds of napiergrass spread widely. It causes diverse germplasms of napiergrass in the field. In this experiment, 104 samples of native napiergrass germplasms were collected from 15 areas in Taiwan. The genetic diversity of these napiergrass germplasms were determined by random amplified polymorphic DNA (RAPD) analysis. The results showed that 94.18% (p<0.001) variance within the population was significantly different by analysis of molecular variance (AMOVA). The hereditary-distance was ranged from 0.0003 to 0.3 among the 15 areas. With the herediary-distance of 0.03, the 104 napiergrass germplasms could be divided into 7 groups: the 1st group from Taipei and Hsinchu, the 2nd group from Miaoli, the 3rd group from Taoyuan, the 4th group from Taitung and Pingtung, the 5th group from Taichung, Nantou, Hualien, Changhua and Yunlin, the 6th group from Tainan, Chaiyi and Kaohsiung, and the 7th group from Eilan. The correlation coefficient between the genetic distance and the cluster dendrogram was 0.68. Taken together, the results suggested that napiergrass lines from the 7 groups might be sufficient for further evaluation to select the elite lines within a shorter period. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。