頁籤選單縮合
題名 | 醫學影像儲傳系統網路斷線管理與備援應變機制於醫學影像相關部門之研究=The Study of Picture Archiving and Communication System Network Disconnection Management and Backup Institution in the Departments of the Medical Imaging |
---|---|
作者姓名(中文) | 林鼎裕; 劉秋霞; 黃武達; 杜俊元; 楊斐適; | 書刊名 | 臺灣應用輻射與同位素雜誌 |
卷期 | 6:1 2010.03[民99.03] |
頁次 | 頁839-844 |
分類號 | 419.21 |
關鍵詞 | 醫學影像儲傳系統; 醫學影像儲傳系資訊安全應變作業標準程序; 放射科資訊系統; 醫院資訊管理系統; Picture Archiving and Communication System; PACS; PACS of information security standards for emergency operating procedures; Radiology Information System; RIS; Hospital Information management System; HIS; |
語文 | 中文(Chinese) |
中文摘要 | 【目的】建立快速检测狐阴道加德纳氏菌的PCR诊断方法。【方法】根据阴道加德纳氏菌16S rRNA基因序列设计1对特异性引物。以分离的狐阴道加德纳氏菌菌体DNA为模板进行PCR扩增,建仁狐阴递加德纳氏菌的PCR检测方法,并对该方法的特异性、敏感性及其可行性进行检验。【结果】特异性试验结果表明,狐阴道加德纳氏菌能扩增出437bp的特异性核酸片段,而大肠杆菌、沙门氏菌、绿脓杆菌、金黄色葡萄球菌的扩增结果均为阴性。敏感性试验结果表明,PCR的最低检出限从为500mL^(-1)。利用建立的PCR检测方法,对9株从山东省不同地区分离的疑似狐阴道加德纳氏菌菌株进行检测,结果有4株为阳性。【结论】研究建立的孤阴道加德纳氏菌PCR决速诊断方法,敏感性高、特异性强,可1次检测多个样品。 |
英文摘要 | 【Objective】 PCR diagnostic method of rapid detection of Gardnerella vaginalis of fox was established. 【Method】 On the basis of the Gardnerella wag mafia 16S rRNA gene, one pair of primers were designed. It took the separated Gardnerella vaginalis of fox DNA as a template and went on PCR amplification. So PCR detection method of Gardnerella vaginalia of fox wail established, and its with specificity and sensitivity were studied. 【Result】 It showed by the specific test results that Gardnerella vaginalia of fox can amplify 437bp specific DNA fragment. However, the amplification results of E. coli, Salmonella pullorum, Bacillus pyocyaneus, Staphylococcus were amplified negatively. The result of the sensitiveities test showed that at least 500ml^(-1) Gardnerella vaginalis of fox was detected with the approach. With the approach of the established PCR the 9 suspected strains isolated from different regions of Shandong Province were detected and 4 strains were positive. 【Conclusion】 The PCR diagnostic method of rapid detection of Gardnerella vaginalis. of fox is successfully established, and its sensitive and can detect more samples at are time. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。