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題名 | 鼻咽癌強度調控放射治療之輻射劑量與誘發二次癌風險評估=Estimation of Doses and Secondary Cancer Risks of the Nasopharyngeal-Carcinoma Intensity-Modulated Radiotherapy |
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作者 | 余婉瑄; 許芳裕; 游澄清; 劉幕台; 黃勝賢; 鐘兆麒; 江崑輝; 林廣軒; | 書刊名 | 臺灣應用輻射與同位素雜誌 |
卷期 | 6:1 2010.03[民99.03] |
頁次 | 頁831-837 |
分類號 | 416.36 |
關鍵詞 | 強度調控放射治療; 二次腫瘤; 風險; 侖道假體; IMRT; Secondary cancer; Risk; Rando phantom; |
語文 | 中文(Chinese) |
中文摘要 | 【目的】建立Ⅰ型鸭病毒性肝炎(Duck hepatitis virus,DHV)的反向遗传系统。【方法】根据Ⅰ型鸭肝炎病毒(DHV-I)ZJ-V/2006株全基因组序列设计并合成5对特异引物,进而应用RT-PCR技术分5段扩增了DHV全基因组cDNA。将扩增的cDNA重叠片段A、B、C和DE片段分别克隆到载体pBluescript Ⅱ KS(+)中,获得了DHV-ⅠZJV/2006株全长基因组cDNA克隆pBIDHV。在扩增5'末端时,引入ApaⅠ酶切位点和SP6启动子序列;在基因组3'米段PolyA尾引入NruⅠ酶切位点,以供cDNA模板的线性化之用。【结果】核酸序列分析表明,DHV-ⅠZJ-V/2006株基因组全长为7711个核苷酸,与DHV-ⅠZJ-V BHK-21细胞分离株的同源性为99.9%;全长基因组中有6个核苷酸发生突变,均未导致对应的氨基酸发生改变,为沉默突变【结沦】成功构建了DHV-ⅠZJ-V/2006株基因组全长cDNA。 |
英文摘要 | 【Objective】 The study was to develop a reverse genetics system of Duck Virus Hepatitis Type Ⅰ (DHV-Ⅰ), 【Method】 Five pairs of oligonticleotides were designed based on the full length genomic sequence of DHV ZJ-V strain, Using RT-PCR technique, five overlapping cDNA fragments, designated as A, B, C, D and E were amplified respectively, And D and E fragments were fused by PCR designated as ED. Using pBluescript Ⅱ KS (+) as a plasmid vector, the full-length cDNA clone pBLDHV of DHV ZJ-V strain was obtained by connecting the four cDNA fragments utilizing single restriction endonuclase site, A Apa Ⅰsite and a SP6 promoter were introduced immediately upstream of 5' end, while a Nru Ⅰ Site was engineered down stream of 3' end of DHV poly (A) tail (containing 20 As). 【Result】 The results of sequencing and analysis showed that there were 99.9% identical between the construction of the full length cDNA sequences and cDNA sequences of DHV-Ⅰ ZJ-V strain, The only differences in sequence were at 6 positions, none of which affected the amino acid sequence. 【Conclusion】 Successful construction of full length cDNA clone of DHV-Ⅰ ZJ-V strain lays a foundation for rescuing DHV effectively and enables further research of DHV at molecular level. |
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