頁籤選單縮合
題 名 | Reciprocal Changes of Renal Neuronal Nitric Oxide Synthase-α and-β Associated with Renal Progression in a Neonatal 5/6 Nephrectomized Rat Model |
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作 者 | Tain, You-lin; Ghosh, Sid; Krieg, Richard J.; Baylis, Chris; | 書刊名 | Pediatrics and Neonatology |
卷 期 | 52:2 2011.04[民100.04] |
頁 次 | 頁66-72 |
分類號 | 417.517 |
關鍵詞 | Chronic kidney disease; Neuronal nitric oxide synthase; Remnant kidney; |
語 文 | 英文(English) |
英文摘要 | Background:Nitric oxide (NO) deficiency contributes to chronic kidney disease progression. NO deficiency could occur for many reasons, one of which is decreased NO synthase (NOS) abundance and/or activity. Methods:In these experiments, we studied two groups of male Sprague Dawley rats given sham or surgical excision of both poles of the left kidney (at 2 days of age) followed by sham or surgical removal of the right kidney at 10 days. Rats were sacrificed at 9 weeks of age and the kidneys examined for abundance of neuronal NOS (nNOS)-α and -β, endothelial NOS, arginase II, argininosuccinate synthase and lysate, protein arginine methyltransferase 1, dimethylarginine dimethylamino-hydrolase 1 and 2, as well as renal pathology. Results:The 5/6 nephrectomy (NX) group showed renal dysfunction, severe rapidly progressing glomerulosclerosis, and hypertension. Renal cortical nNOSα abundance was significantly reduced, whereas nNOSβ abundance was increased in the 5/6 NX group versus sham. Renal endothelial NOS was unchanged. Next, renal protein arginine methyltransferase 1 abundance was higher, whereas dimethylarginine dimethylamino-hydrolase 2 expression was lower in the 5/6 NX group versus sham. Renal arginase II, argininosuccinate synthase, and argininosuccinate lysate abundances were significantly decreased in 5/6 NX rats than those in sham. Conclusion:The neonatal kidney is very susceptible to 5/6 NX-induced injury, and, as in adults, reciprocal changes in the nNOSα and nNOSβ in renal cortex occur during progression of chronic kidney disease and may contribute to the injury. |
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