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頁籤選單縮合
題名 | 808nm砷鋁化鎵半導體雷射照射對試管內人類肝癌細胞生長產生抑制作用及其可能的機轉=The Inhibition Effect and Related Mechanism of Diode 808nm GaAlAs Laser on the Proliferation of Human Carcinoma Cells |
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作者姓名(中文) | 劉奕祥; 劉文清; 過傳良; 陳志豪; | 書刊名 | 中華民國口腔雷射醫學雜誌 |
卷期 | 2:1 民95.11 |
頁次 | 頁7-18 |
分類號 | 416.246 |
關鍵詞 | 低能量雷射照射; 肝癌; 細胞骨架; 細胞角質分子; 中間型絲; 能量密度; Low-power laser irradiation; Hepatocellular carcinoma; Cytoskeleton; Cytokeratin; Intermediate filament; Power density; |
語文 | 中文(Chinese) |
中文摘要 | 肝癌在台灣十分常見,在男性,最常見致死的癌症為肝癌,而對於女性,它也高居第二位,可見肝癌對國人健康造成極大威脅。「低能量雷射照射」在醫療領域已經是一種被廣泛運用的工具,但是有關低能量雷射照射對人類肝細胞及肝癌細胞之影響的研究卻非常少。在我們過去的研究中,我們發現人類肝細胞的細胞角質分子在肝癌發生的過程中會發生改變,因此,我們認為一個不安定的細胞骨架與腫瘤的發生必有密切關聯。在這一次的研究中,我們企圖探討低能量雷射照射是否會影響人類肝癌細胞之細胞骨架安定性。此外我們也想了解人類肝癌細胞的生長是否受低能量雷射照射影響。結果顯示細胞數目、PCNA和Histone的表現減少,顯示出HepG2及J-5細胞的細胞增生是受到低能量雷射照射的抑制。對HepG2和J-5細胞影響最大的照射時間分別是90和120秒(能量密度分別是5.85J/平方公分和7.8J/平方公分),在這劑量的照射下,HepG2和J-5細胞的細胞減少率分別為76%和66%。除此之外,雷射照射後這些細胞微小管和中間型絲結構的完整性也受至破壞,中間型絲相關蛋白Plectin和synemin的表現也被減少了。本研究提出兩個有意義的發現:(1)808 nm GaAlAs半導體雷射照射會抑制人類肝癌細胞株HepG2和J-5的細胞數。(2)進一步分析808 nm GaAlAs半導體雷射抑制人類肝癌細胞生長之淨效應。這個抑制作用的機轉可能是由於Plectin和synemin等中間型絲相關蛋白的表現受雷射照射之影響而下降,不足的Plectin和synemin則造成微小管和細胞角質分子結構的改變而失去支撐細胞的功能。 |
英文摘要 | Low-power laser irradiation (LPLI) has come into a wide range of use in medical field, but only few data about the effects of LPLI on human liver or hepatoma cells were studied. The stability of cytokeratin molecules in human hepatocytes was related with the intact microtubule network that was influenced by colchicines had been proved. In this study, we are going to search the effect of LPLI on proliferation of human hepatoma cell line HepG2 and J-5 cells. In addition, the stability of microtubule and cytokeratin as well as plectin and synemin (one of the intermediate filament-associated proteins) were analyzed under the action of LPLI to evaluate the possible mechanism of LPLI effects on proliferation of human hepatoma cells. The results revealed that proliferation of HepG2 and J-5 cells was inhibited by LPLI since the cell counts, PCNA and histone expression was reduced. The decreased ratio of cell number by this dose of irradiation was 72% and 66% in HepG2 and J-5 cells, respectively. Besides that, the architecture of microtubule and intermediate filaments in these cells was disorganized by laser irradiation. The expression of intermediate filament-associated protein, synemin and plectin, was also reduced. We concluded that diode 808 nm GaAlAs continue wave laser has an inhibitory effect on the proliferation of human hepatoma cell line HepG2 and J-5. Eventually, cell integrity and morphology cannot be maintained and this might cause hepatoma cell death. |
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