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題 名 | Possible Mechanism of Betel-quid-extract-induced Expression of Matrix Metalloproteinase-2 |
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作 者 | Liu, Yu-chi; Lin, Mei-huei; Liu, Shyun-yeu; Chiang, Wei-fan; Chen, Li-lin; Chen, Tai-chi; Cheng, Yon-chi; Hsu, Kai-chen; Cheng, Pse-chou; Lee, Chin-hai; Liu, Young-chau; | 書刊名 | Journal of the Formosan Medical Association |
卷 期 | 109:11 2010.11[民99.11] |
頁 次 | 頁838-847 |
分類號 | 416.94 |
關鍵詞 | Betel quid; Matrix metalloproteinase-2; |
語 文 | 英文(English) |
英文摘要 | Background/Purpose: Betel quid extract (BQE) has been demonstrated to induce matrix metalloproteinase (MMP)-2 expression. This study aimed to establish the possible mechanism involved in this event. Methods: Western blotting, reverse-transcription polymerase chain reaction, and gelatin zymography were used to study the expression level of MMP-2. LY294002, PD98059, U0126, N-acetyl-L-cysteine, SB203580, SP600125, and Bay 11-7082 were used to pretreat OECM-1 cells before BQE treatment and MMP-2 detection. Results: OECM-1 cells were subjected to short-term (10 minutes) or long-term (24 hours) BQE treatment (designated as SBT and LBT, respectively), and we found that both treatments increased MMP-2 protein and extracellular signal-regulated kinase (ERK) phosphorylation levels in a concentration- and time-dependent manner. LBT also increased MMP-2 mRNA level. LBT-induced MMP-2 secretion was not inhibited by PD98059 (up to 50 μM) when ERK was effectively blocked, but was attenuated by LY294002 (0–10 μM) in a concentrationdependent manner. This LBT effect was inhibited strongly by SB203580 (10 μM), SP600125 (10 μM), and Bay 11-7082 (10 μM) and mildly by N-acetyl-L-cysteine (5 mM), but not by U0126 (10 μM). Conclusion: Both SBT and LBT upregulate MMP-2 expression, and LBT-induced MMP-2 expression might be mediated by phosphoinositide 3-kinase, p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-κB, and to a lesser extent, by reactive oxygen species, rather than by ERK. |
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