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題名 | 耐力訓練對骨骼肌肌纖維類型與細胞凋亡關係的影響=Effect of Endurance Exercise Training on the Relationship between Myosin Heavy Chain Isoforms and Apoptosis in Skeletal Muscle |
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作者姓名(中文) | 陳聰毅; 林念璁; | 書刊名 | 體育學報 |
卷期 | 43:3 2010.09[民99.09] |
頁次 | 頁17-34 |
分類號 | 528.9012 |
關鍵詞 | 耐力運動; 重鏈肌凝蛋白; 骨骼肌; 細胞凋亡; 基因表現; Endurance exercise; Myosin heavy chain; Apoptosis; Skeletal muscle; mRNA expression; |
語文 | 中文(Chinese) |
中文摘要 | 目的:探討耐力運動訓練後,腓腸肌(gastrocnemius)與比目魚肌(soleus)不同重鏈肌凝蛋白(myosin heavy chain, MyHC)異構物與細胞凋亡現象及粒腺體訊息傳遞分子mRNA基因表現量的關係。方法:將8週齡雄鼠(Sprague-Dawley)隨機分成控制組(n=6)及運動組(n=6),運動組在動物跑步機上進行10週之耐力運動訓練。結果:耐力運動訓練增加腓腸肌及比目魚肌之檸檬酸合成酶活性(p<.05);腓腸肌增加MyHC I、MyHC IIa與MyHC IIx及減少MyHC IIb的比率(p<.05),比目魚肌增加MyHCI及減少MyHC IIa的比率(p<.05);減少腓腸肌與比目魚肌之細胞凋亡現象(p<.05),而且比目魚肌細胞凋亡現象低於腓腸肌(p<.05);腓腸肌增加B細胞淋巴瘤/白血病2(B-cell leukemia/lymphoma2, Bcl-2)及減少細胞凋亡誘發因子(apoptosis inducing factor, AIF) mRNA表現量(p<.05),比目魚肌增加Bcl-2、Bcl-2-性關聯蛋白(Bcl-2-associated X protein, Bax)與熱休克蛋白70(heat shock protein70, HSP70)及減少劊白酶9(caspase-9)與AIF等mRNA表現量(p<.05)。結論:(一)耐力運動訓練增加腓腸肌及比目魚肌之MyHC I比率,與骨骼肌細胞凋亡現象及其粒腺體訊息傳遞分子mRNA基因表現量下降有關,此現象對比目魚肌的影響大於腓腸肌。(二)耐力運動訓練後之比目魚肌,除了可降低粒腺體誘發細胞凋亡訊息傳遞分子mRNA基因表現量外,同時也會增加抗細胞凋亡分子之mRNA基因表現量,在腓腸肌無此現象。 |
英文摘要 | To examine the relationship between myosin heavy chain (MyHC) isoforms and apoptosis in the gastrocnemius and soleus after endurance exercise training. Methods: Male Sprague-Dawley rats were randomly assigned to an untrained (CON, n=6) or an endurance training group (ET, n=6). The ET exercised on a treadmill for 10 weeks. Results: After endurance exercise traning, citrate synthase activity was increased in both gastrocnemius and soleus (p<.05). In gastrocnemius, the content of MyHC I, MyHC IIa and MyHC IIx were increased while MyHC IIb was decreased (p<.05). In soleus, the content of MyHC I was increased while MyHC IIa was decreased (p<.05). Apoptotic index was decreased in both gastrocnemius and soleus. Also, the apoptotic index of soleus was lower than the gastrocnemius after endurance training (p<.05). B-cell leukemia/lymphoma 2 (Bcl2) mRNA was up-regulated while apoptosis inducing factor (AIF) mRNA was down-regulated in gastrocnemius of ET. Bcl-2, Bcl-2-associated X protein (Bax) and heat shock protein 70 (HSP 70) mRNA were up-regulated while caspase-9 and AIF mRNA were down-regulated in soleus of ET. Conclusions: 1) Decreased apoptosis and down-regulated the mRNA expression of mitochondrial dependent apoptotic signaling were correlated with that the content of MyHC I increased in both gastrocnemius and soleus after endurance exercise training that is more sensitive to influence soleus than gastrocnemius. 2) Endurance exercise training not only down-regulated the mRNA of mitochondrial dependent apoptotic signaling but up-regulated the mRNA of anti-apoptosis in soleus that didn't occur in gastrocnemius. |
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