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題 名 | Supercritical Fluids Extraction of Cinnamic Acid Derivatives from Brazilian Propolis and the Effect on Growth Inhibition of Colon Cancer Cells |
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作 者 | Chen, Chao-rui; Lee, Ying-nong; Lee, Miau-rong; Chang, J. Chieh-ming; | 書刊名 | Journal of the Taiwan Institute of Chemical Engineers |
卷 期 | 40:2 2009.03[民98.03] |
頁 次 | 頁130-135 |
分類號 | 460.02 |
關鍵詞 | SC-CO₂ extraction; Propolis; DHCA; Column adsorption; Growth inhibition; Colon cancer cells; |
語 文 | 英文(English) |
英文摘要 | Abstract This work examined supercritical carbon dioxide (SC-CO2) extractions of an anti-cancer compound, 3,5-diprenyl-4-hydroxycinnamic acid (DHCA) from propolis lumps and studied the inhibitive effect of the SC-CO2 extracts on the growth of two cancer cells. The maximum amount of DHCA, 91.9 mg/g, was obtained by Soxhlet ethyl acetate extraction and the 41.2 wt.% pure DHCA was recovered using SC-CO2 at 20.7 MPa and 323 K with 6 wt.% ethyl acetate addition. The effects of temperature and the addition of ethyl acetate on the DHCA purity, examined using a two factorial central composite response surface methodology, indicated that both factors were significant. A normal phase column adsorption approach was directly employed to purify the DHCA, and the purity was increased to 95 wt.%. The cell concentrations following growth inhibition ranged from 10 μg/mL to 500 μg/mL, indicating that the SC-CO2 extracts and 95 wt.% DHCA effectively inhibited the growth of human leukemia (HL-60) and colon (colo 205) cancer cells, but did not suppress the growth of two human normal cells. |
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