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題名 | Development of a Molecular Method for Rapid Differentiation of Watermelon Lines Resistant to Fusarium Oxysporum f. sp. niveum=西瓜抗蔓割病品系之快速分子檢測技術之開發 |
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作者 | 林盈宏; 陳甘澍; 劉政道; 黃振文; 張碧芳; Lin, Ying-hong; Chen, Kan-shu; Liou, Tsung-dao; Huang, Jenn-wen; Chang, Pi-fang Linda; |
期刊 | Botanical Studies |
出版日期 | 20090700 |
卷期 | 50:3 2009.07[民98.07] |
頁次 | 頁273-289 |
分類號 | 435.264 |
語文 | eng |
關鍵詞 | 分子標誌; 分子檢測; 西瓜; 西瓜蔓割病菌; 育種; 聚合酵素連鎖反應; 隨機增幅核酸多型性分析; 鐮孢菌萎凋病; SCAR; Breeding programs; Citrullus lanatus; Fusarium oxysporum f. sp. niveum; Fusarium wilt; Molecular detection; Molecular marker; PCR; RAPD; |
中文摘要 | 西瓜蔓割病係由 Fusarium oxysporum f. sp. niveum 所引起,為世界西瓜生產的重要限制因子之一。本病屬於維管束萎凋病,防治頗為困難,惟目前最好的防治方法即是選育及栽培抗病品種(系)。為提昇西瓜抗蔓割病選育之效率,本研究利用聚合酵素連鎖反應 (polymerase chain reaction) 技術搭配 GsF5/ GsR5 引子對,用以快速判別抗病之西瓜品系。GsF5/GsR5 係由 OP-G05 隨機引子之增幅片段序列配合 SCAR (sequence characterized amplified region) 技術所設計之引子對,其增幅出之 898 bp 核酸片段對抗蔓割病之西瓜品系具專一性。本分子檢測法不僅容易快速判別西瓜雜交族群對蔓割病菌之不同感受性外,尚可用於判別西瓜商業品種的抗病與感病性。 |
英文摘要 | The vascular wilt disease of watermelon caused by Fusarium oxysporum f. sp. niveum (Fon) is one of the major factors limiting watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) production worldwide. Breeding highly resistant lines is one of the best ways to control the disease. In order to accelerate the breeding programs, a reliable PCR (polymerase chain reaction) technique was developed to rapidly differentiate the disease-resistant watermelons. The G05-SCAR (sequence characterized amplified region) primer set GsF5/GsR5, derived from the OP-G05 random primer-amplified fragment sequence, produced an 898 bp DNA fragment which was specific to watermelon lines resistant or tolerant to Fon. With optimized PCR assay, the molecular method could easily differentiate Fon-resistant and -susceptible hybrid watermelon populations. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。