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題名 | 野茼蒿(Conyza sumatrensis)抗巴拉刈生物型之抗氧化活性研究=Antioxidant Activity of Paraquatresistant Biotype of Tall Fleabane (Conyza sumatrensis) |
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作者姓名(中文) | 蔣永正; 王智屏; 蔣慕琰; | 書刊名 | 作物、環境與生物資訊 |
卷期 | 6:4 2009.12[民98.12] |
頁次 | 頁209-219 |
分類號 | 433.85 |
關鍵詞 | 野茼蒿; 巴拉刈; 抗性生物型; 抗氧化活性; Tall fleabane; Conyza sumatrensis; Paraquat; Resistant biotype; Antioxidant activity; |
語文 | 中文(Chinese) |
中文摘要 | 摘要 以葉面積及植株鮮重之50%抑制濃度, 篩選出臺灣地區對巴拉刈具抗性與感性之野 茼蒿生物型(抗感比為16-30 倍)。抗、感植株 葉圓片浸置於不同濃度之巴拉刈溶液中,照 光4 小時後,葉片中MDA 含量均隨處理濃 度提高而增加,但抗性植株之累積速率較感 性植株明顯為低。抗性植株葉片中之抗壞血 酸(ascorbate)在藥劑未處理前之含量較感性 葉片高約60%。感性植株在25 μM 低濃度處 理下,抗壞血酸含量快速下降約52%,抗性 植株在處理濃度提高至100 μM,抗壞血酸含 量僅減少24%左右。藥劑處理前之抗性與感 性葉片中穀胱苷肽(glutathione)含量相近,且 均隨巴拉刈處理濃度的提高而明顯減少,但 感性植株在低濃度25 μM 處理下快速減少約 86%,較抗性植株之下降速率顯著為高。巴 拉刈處理後抗性與感性葉片中抗壞血酸及穀 胱苷肽含量減少時,MDA 累積量則有增加之 趨勢。以不同濃度之巴拉刈處理抗、感植株 葉圓片,感性植株於25 μM 巴拉刈濃度下, DPPH 自由基清除力快速降低至40%以下, 抗性植株在25-100 μM 濃度處裡下,仍維持 90%以上之自由基清除力。測定抗、感性植 株葉圓片之總酚含量、還原力及螯合亞鐵離 子能力,顯示感性葉片中之相關反應會明顯 受到藥劑的抑制。以50 μM 巴拉刈處理野茼 蒿植株,抗、感生物型中總酚含量、還原力 及螯合亞鐵離子能力之比值,均較藥劑處理 前之比值明顯提高,推測抗性生物型中清除 自由基之抗氧化能力可能與降低巴拉刈毒性 之抗性機制有關。 |
英文摘要 | ABSTRACT A tall fleabane (Conyza sumatrensis) biotype resistant to paraquat was found in upland fields in Taiwan. Based on the reduction of leaf area and plant fresh weight of remaining green evaluated 7 days after paraquat application, the concentration required to achieve 50% inhibition of the resistant (R) biotype was about 16-30 times than that of the susceptible (S) one. Leaf disc assay of R- and S-biotypes, treated with paraquat at different concentrations for four hours, showed a faster accumulation of malondialdehyde (MDA) in S-biotype than in R-biotype after exposed to paraquat. The content of ascorbate was higher in R-biotype, but glutathione contents were similar in both biotypes. Observation on the levels of ascorbate and glutathione after paraquat treatment indicated that this oxidative treatment principally induced a marked reduction ofascorbate and glutathione pools in S-biotype. The MDA accumulation in S-biotype subjected to paraquat treatment could be attributed to the loss of ascorbate and glutathione. In the DPPH assay, R-biotype had higher free radical scavenging capacity than S-biotype after 25-100 μM paraquat treatments. The multiple antioxidant activity of R-biotype was evident with significant total phenol content, reducing power and ferrous ion chelating potency. The results suggested that the observed paraquat resistance may partly be attributed to the antioxidant activity. |
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