查詢結果分析
來源資料
頁籤選單縮合
題名 | 水稻抗白葉枯病基因表現蛋白質之多白胺酸重複(LRR)的分布及胺基酸組成=Leucine-rich Repeat (LRR) Distribution and Amino Acid Composition in Protein Sequences of Genes with Resistance to Bacterial Blight (Xanthomonas oryzae) of Rice |
---|---|
作者 | 林奕承; 呂椿棠; 魏夢麗; 呂秀英; | 書刊名 | 臺灣農業研究 |
卷期 | 58:2 2009.06[民98.06] |
頁次 | 頁93-105 |
分類號 | 434.111 |
關鍵詞 | 水稻; Xa基因; 白葉枯病; 多白胺酸重複; 胺基酸組成及屬性; Rice; Xa genes; Bacterial blight; Xanthomonas oryzae pv. oryzae; Leucine-rich repeat; Amino acids; |
語文 | 中文(Chinese) |
中文摘要 | 目前已知多白胺酸重複(leucine-rich repeat, LRR)為生物體抗病基因蛋白質序列上的特有序列,因此本研究針對水稻所有已定序之Xa基因,自NCBI(National Center for Biotechnology Information)及KOME(Knowledge-based Oryza Molecular Biological Encyclopedia)公共資料庫內擷取出它們已知的CDS(coding domain sequence)與全長cDNA序列,將其轉譯的蛋白質序列利用Pfam蛋白質家族資料庫尋找出各LRR片段,發現僅在Xa1、Xa21及Xa26基因之表現蛋白質結構中發現LRR存在。進而利用生物資訊與統計方法,進行不同Xa基因蛋白質之LRR序列的分布及胺基酸組成之比較分析。結果顯示,Xa1之LRR在蛋白質序列中鬆散分布於NB-ARC(nucleotidebinding adaptor shared by APAF-1, R proteins, and CED-4)區域序列之後,而Xa21及Xa26之LRR則密集分布在NB-ARC或P-kinase(protein-kinase)區域序列之前。Xa1的蛋白質序列中靠近NB-ARC區域序列的前一、二個LRR似乎較遠離其他LRR,但Xa21和Xa26的蛋白質之LRR區域結構並未有如此現象。三個Xa基因表現蛋白質之LRR序列的胺基酸組成皆以親水性胺基酸為最多且其中以白胺酸(leucine)出現頻率最高,其次為不帶電荷之絲胺酸(serine)。Xa1蛋白質之LRR在某些胺基酸的出現頻率相異於Xa21和Xa26。這些結果有助於了解不同Xa基因間表現蛋白質中特有LRR序列的結構變異情形。 |
英文摘要 | Since leucine-rich repeat (LRR) is known as a domain of protein specific to disease resistance genes, it is used to study the genes of rice with resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae. The CDS (coding domain sequence) and full-length cDNA sequences of the completely sequenced genes were obtained through the access from public databases of NCBI (National Center for Biotechnology Information) and KOME (Knowledge-based Oryza Molecular Biological Encyclopedia) and they were used to identify each LRR fragment in translated protein sequences through Pfam protein families database. Results showed that only Xa1, Xa21 and Xa26 genes were found to possess LRRs in their protein sequences. When bioinformatics and statistical methods were used for further comparative analysis of distribution and amino acid composition for these LRRs among Xa protein sequences, LRRs of Xa1 were loosely located after NB-ARC (nucleotide-binding adaptor shared by APAF-1, R proteins, and CED-4) in the protein sequences, whereas LRRs of Xa21 and Xa26 were densely located before NB-ARC or P-kinase (protein-kinase). It appeared that the first one or two LRRs of the gene Xa1 proteins in proximity to NB-ARC were distant from the other LRRs, but no similar phenomenon was observed within the protein sequences of the Xa21 and Xa26 genes. The LRRs of these three Xa genes mainly consisted of hydrophilic amino acids, with the highest frequency for leucine, followed by neutral serine. The LRRs of Xa1 protein sequences had different frequencies for some amino acids compared to LRRs of Xa21 and Xa26. These findings are useful in understanding of the structural variation of LRRs in protein sequences among Xa genes with resistance to bacterial blight of rice. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。