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題名 | Characterization of the Response of Dendritic Cells and Regulatory T Cells to Tumor Antigens in Patients with Renal Cell Carcinoma=腎細胞癌患者對腫瘤抗原之免疫反應 |
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作者 | 侯明模; 張文震; 馮思中; 江仰仁; 沈永吉; 廖順奎; 謝佳娟; 葉光揚; 張乃仁; 莊正鏗; | 書刊名 | 長庚醫誌 |
卷期 | 33:1 2010.01-02[民99.01-02] |
頁次 | 頁25-35 |
分類號 | 415.138 |
關鍵詞 | 樹突狀細胞; 調節型T細胞; 周邊血液; 腎細胞癌; Dendritic cells; Regulatory T cells; Peripheral blood; Renal cell carcinoma; |
語文 | 英文(English) |
英文摘要 | Background: This study characterized dendritic cells (DCs), regulatory T cells (Tregs) and the immune responses to tumor antigens in renal cell carcinoma (RCC) patients. Methods: Thirty patients with RCC and five healthy donors were studied. DCs were generated from the adherent cells among peripheral blood mononuclear cells (PBMCs), then cultured in medium containing granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 for 7 days. The phenotypes of the DCs and Tregs were analyzed by flow cytometry. A mixed lymphocyte reaction (MLR) was performed to assess the functioning of the DCs and Tregs. A cytotoxic assay was performed to measure the antigen presentation ability of the DCs from the RCC patients (RCC-DCs). These DCs were pretreated with TNF-α (TNF-DCs) or tumor lysate (TuLy-DCs) on the 3rd day of DC culture. Results: The RCC-DCs expressed significantly less CD40 (p = 0.03) and CD80 (p = 0.007) upon TNF-α cultivation than the DCs from healthy donors. The peripheral Tregs during stage I disease were significantly less (p = 0.032) than during stages II-IV. The RCC-DCs were as efficient as DCs from healthy donors (p = 0.83) when stimulating the proliferation of allogeneic T cells; however, these RCC-DCs were less efficient when stimulating autologous T cells than allogeneic T cells (p = 0.023). Tregs inhibited autologous T cell proliferation rather than allogeneic T cell proliferation in response to TuLy-DCs stimulation. Prostaglandin E2 did not increase the ability of immature DCs to stimulate T cell proliferation. Conclusions: Patients with RCC have less potent anti-tumor immune responses. |
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