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相關文獻
- 孵化場絨毛所分離之沙門氏菌抗生素抗藥性之變化
- Antibiotic Resistant Switch in Salmonellae Isolated from Chicken Hatchery Fluff in Taiwan
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頁籤選單縮合
題名 | Antibiotic Resistant Switch in Salmonellae Isolated from Chicken Hatchery Fluff in Taiwan=孵化場絨毛分離之沙門氏菌抗生素抗藥性變化 |
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作者 | 洪惠雯; 邱怡昌; 王金和; Hong, Hui-wen; Chiu, Jimmy; Wang, Ching-ho; |
期刊 | 臺灣獸醫學雜誌 |
出版日期 | 20090300 |
卷期 | 35:1 2009.03[民98.03] |
頁次 | 頁9-14 |
分類號 | 437.243 |
語文 | eng |
關鍵詞 | 抗藥性基因; 沙門氏菌; Antibiotic resistance; Integron; Salmonella; |
中文摘要 | 本篇研究調查西元2002-2003年及2006年之間,由台灣各地禽類孵化場絨毛隨機分離出的55株沙門氏菌之抗藥性基因與integron變化情形。integron為近幾年來被認為繼抗藥性質體(plasmid)和跳躍基因(transposon)之外,另一種與細菌獲得新的抗藥性基因和傳遞有關的機制。本研究以聚合酵素連鎖反應(polymerase chain reaction, PCR)偵測抗藥性基因、與integron之變化,並以統計分析。結果顯示integron廣泛存在於沙門氏菌中,大小介於0.6kb至2.3kb之間。在2002-2003年與2006年這兩組中,tetA、tetB及PSE的分佈具有統計上的意義(p<0.01);此外,即便在同一場中所分離的沙門氏菌在抗藥性基因組成上亦有所差異,推測integron可能於細菌之間互相傳遞交換。而本研究同時也顯示帶有integron的沙門氏菌株擁有兩種以上抗藥性基因之比例相對較高。綜合以上,integron於臨床沙門氏菌中普遍存在並攜帶抗藥性基因,影響其抗藥性變化。 |
英文摘要 | In order to monitor salmonella contamination in chicken hatcheries during 2001 to 2007, fluff samples collected from different hatcheries in Taiwan were running bacterial isolation for resistant gene analysis. The salmonella isolation rates for the collected samples ranged from 0% to 4% in different years. From which, 55 salmonella isolates in 2002 and in 2006 were selected for the analysis of the resistant gene coding for integron, tetA, tetB, and PSE-1 by using polymerase chain reactions. These isolates contained more tetA and PSE-1 genes but less tetB gene in 2002 than in 2006. However, the presence of integron in 2002 and 2006 was 70% and 64%, respectively. The high existence of integron in the local isolates of salmonellae reveals a high risk of antibiotic resistant genes transferring by this element among different salmonellae. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。