查詢結果分析
來源資料
相關文獻
- 愛文芒果(Mangifera Indica L. cv. Irwin)類胡蘿蔔素生合成基因在果實發育時期之基因表現分析與選殖
- 酚類資化酵母菌Candiada tropicalis酚類羥化酶基因的選殖
- Carotenoid Content in Various Tissues of Cultured Penaeus monodon by Their Sizes, Sexes, and Molting Stages
- 青芒果調製一貫自動化作業機械之研製及改進
- 植物基因轉殖之研究
- 芒果醋之釀造
- 不同品種芒果砧木對無機元素吸收運移能力之探討
- 芒果果粒大小與荷重關係之探討
- 藻類(Haematococcus)做為天然呈色劑
- 牛傳染性鼻氣管炎病毒封套醣蛋白gC基因之選殖與定序
頁籤選單縮合
題名 | 愛文芒果(Mangifera Indica L. cv. Irwin)類胡蘿蔔素生合成基因在果實發育時期之基因表現分析與選殖=Cloning of Phytoene Desaturase Gene in Mango (Mangifera Indica L. cv. Irwin) and Its Expression at Different Stages of Fruit Development |
---|---|
作者姓名(中文) | 李文立; 邱國棟; 林榮貴; 陳福旗; | 書刊名 | 臺灣農業研究 |
卷期 | 57:3 2008.09[民97.09] |
頁次 | 頁213-229 |
分類號 | 435.325 |
關鍵詞 | 芒果; 類胡蘿蔔素; 八氫茄紅素去氫酶; 基因選殖; Mango; Mangifera indica; Carotenoids; Phytoene desaturase; Gene cloning; Gene expression; |
語文 | 中文(Chinese) |
中文摘要 | 本研究利用快速增殖cDNA (rapid amplification of cDNA ends, RACE)技術,從愛文芒果果皮之訊息RNA(mRNA)中選殖類胡蘿蔔素(phytoene desaturase, PDS)基因之互補DNA(cDNA)以瞭解其果實發育期間色素基因表現情形。PDS基因全長爲1,882bp,包含一個長達1,644bp的解讀框架(open reading frame, ORF)可轉譯成548個胺基酸,5'及3'端未轉譯區(untranslated region, UTR)分別爲36bp及202bp,由序列比對分析結果顯示,該基因的胺基酸序列與其他植物物種具有50.0-74.1%的相似性。PDS基因經逆轉錄及DNA擴增後利用管家基因(housekeeping gene) actin之相對表現量,偵測其在不同果實發育時期之相對表現量,顯示愛文芒果PDS基因在果實發育期間爲穩定表現之基因,PDS基因從幼果期至黃熟果均有表現,與actin基因的相對表現量在幼果期(stage1)最高,隨著果實發育,該基因的表現量呈現降低的趨勢。而果實在成熟過程中,總胡蘿蔔素的含量會在果實達到生理成熟期後才會快速地大量增加,而該基因的表現量卻未隨總胡蘿蔔素的增加而增加,顯示在果實後熟過程中,PDS基因並未對類胡蘿蔔素生成有直接的影響。由於在果實發育過程中,芒果PDS基因的表現量呈現下降之趨勢,該基因的表現模式應爲負調節(down-regulated)方式。 |
英文摘要 | Mango (Mangifera indica) is a tropical fruit containing carotenoids. In order to understand the expression of phytoene desaturase (PDS), a key enzyme for the biosynthesis of β-carotene, during fruit development, we havecloned and characterized a cDNA encoding PDS from young mango fruits, cv. Irwin. Mango PDS cDNA contains 1,882bp, with 1,644bp open reading frame encoding for a polypeptide of 548 amino acid residues. The 5' and 3' untranslated regions (UTR) were 36bp and 202bp, respectively. The deduced amino acid sequence of this PDS has 50.0-74.1% of similarity to PDS from other plant species. The relative expression levels and carotenoids content of the Irwin mango PDS at the six fruit development stages and in mature leaves were detected. The result showed that the expression level of PDS gene at different fruit development stages was reduced and the carotenoids increased rapidly, when fruit are maturing. These findings suggest that mango PDS is down regulated during the fruit ripening process. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。