查詢結果分析
來源資料
頁籤選單縮合
題 名 | 應用生物晶片診斷乳房炎病原菌之研究=Application of Biochip on Classification of Mastitis Pathogen in Cow |
---|---|
作 者 | 李國華; 施意敏; 莊士德; 李素珍; 張菊犁; 吳明哲; 季昭華; | 書刊名 | 畜產研究 |
卷 期 | 40:1 2007.03[民96.03] |
頁 次 | 頁49-57 |
分類號 | 437.243 |
關鍵詞 | 乳房炎; 生物晶片; 乳房炎病原菌; Mastitis; Pathogen; Biochip; |
語 文 | 中文(Chinese) |
中文摘要 | 乳房炎是乳牛最重要的疾病且廣佈於世界各地,造成的經濟損失甚鉅,為達快速防治乳房炎之目的,本試驗建立乳房炎生物晶片之檢測法並應用於泌乳牛群,可在六小時內快速檢出牛乳中之金黃色葡萄球菌(Streptococcus aureus)與無乳鏈球菌(Streptococcus agalactiae)兩種傳染性病原菌,以及大腸桿菌(E. coli)、異乳鏈球菌(Streptococcus dysgalactiae)、乳房鏈球菌(Streptococcus uberis)、牛鏈球菌(Streptococcus bovis)等四種環境性病原菌。採集1ml的生乳,經去乳脂、萃取微生物DNA、PCR反應、DNA雜合反應與呈色反應後,根據晶片上具專一性之核酸探針的點陣排列圖直接判讀結果。在2004年,篩檢207戶泌乳牛群之總乳,牛鏈球菌陽性檢出率最高163戶(78.7%),其次依序為乳房鏈球菌(60.4%)、大腸桿菌(45.4%)、異乳鏈球菌(30.0%)、無乳鏈球菌(27.5%)及金黃色葡萄球菌(9.2%),顯示乳房炎生物晶片是一快速檢測牛乳中乳房炎病原菌的新方法,未來加入乳房炎防治工作深具潛力。 |
英文摘要 | In order to efficiently prevent and treat bovine mastitis and minimize its impact to the dairy industry, a sensitive, rapid, and specific test is required for identifying the mastitis-causing pathogens. In the present study, we used biochip to examined the distribution of mastitis-causing pathogens in Taiwan. The biochip is capable of detecting 6 common species of mastitis-causing pathogens within 6 hours, including Streptococcus bovis, Streptococcus uberis, Streptococcus agalactiae, Streptococcus dysgalactiae, Escherichia coli and Streptococcus aureus. The technique is based on DNA amplification of genes specific to the target pathogens and consists of 4 basic steps: DNA extraction of bacteria, PCR reaction, DNA hybridization, colorimetric reaction. The Biochip was used for detecting bacteria in bulk tank milk samples from 207 DHI-participating dairy farms in 2004. The results show that Streptococcus bovis, detected in samples from 163 (78.7%) farms, was the most prevalent species, followed by Streptococcus uberis (60.4%), Escherichia coli (45.4%), Streptococcus dysgalactiae (30.0%), Streptococcus agalactiae (27.5%), and Streptococcus aureus (9.2%). Results from this study showed that the biochip is a feasible tool for a rapid diagnose of mastitis-causing pathogens in milk, which might provide information for a more effective treatment to cure mastitis. |
本系統中英文摘要資訊取自各篇刊載內容。