頁籤選單縮合
題名 | Application of Restriction Fragment Length Polymorphism of the Region Encoded yba3 and yba4 of Buchnera Aphidicola for Molecular Identification of Aphids=以限制片段長度多型性選殖蚜蟲內共生菌Buchnera Aphidicola yba3-yba4 DNA片段於蚜蟲分子鑑定之應用 |
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作者 | 溫育德; 阮莉芸; 陸維濃; 林天偉; 陳佳郁; 賴吉永; 郭美華; Wen, Yu-der; Juan, Li-yun; Lu, Wei-nung; Lin, Tien-wei; Chen, Chia-yu; Lai, Chi-yung; Kuo, Mei-hwa; |
期刊 | 臺灣昆蟲 |
出版日期 | 20081000 |
卷期 | 28:2 2008.10[民97.10] |
頁次 | 頁123-131 |
分類號 | 385.752 |
語文 | eng |
關鍵詞 | 蚜蟲分子鑑定; 蚜蟲內共生菌; RFLP; Aphids molecular identification; Buchnera aphidicola; Restriction fragment length polymorphism; |
中文摘要 | 本研究目的在於發展出一種新的分子標記用來鑑定蚜蟲的種類。本試驗分別自五 種蚜蟲:黑豆蚜 Aphis craccivora、酸模蚜 Aphis rumicis、棉蚜 Aphis gossypii、 桃蚜 Myzus persicae 及白尾紅蚜 Uroleucon formosanum 體內的共生菌 Buchnera aphidicola 中,選殖出一段含有 B. aphidicola 兩個特有的基因 yba3 及 yba4 的 DNA 片段。接著利用限制片段長度多型性 (RFLP) 的方法,對 yba3-yba4 DNA 片段進行 限制內切酶剪切分析。試驗發現來自五種蚜蟲內共生菌的 yba3-yba4 DNA 片段,經 由 Taq I 酵素剪切後,會產生不同大小的剪切片段。由於蚜蟲內共生菌與寄主蚜蟲有 著絕對共生關係,因此 RFLP 分析 yba3-yba4 DNA 片段的結果,可作為一種分子標 記,用以鑑定不同屬的蚜蟲。 |
英文摘要 | A new molecular marker was developed for the identification of aphids. The regions encoding two species-specific orphan genes yba3 and yba4, from five Buchnera aphidicola strains associated with Aphis craccivora (BAc), Aphis rumicis (BAr), Aphis gossypii (BAg), Myzus persicae (BMp), and Uroleucon formosanum (BUf) were cloned and sequenced. The restriction fragment length polymorphism (RFLP) assay on the yba3-4 regions using the TaqI restriction enzyme produced distinguishable fragments in BAg, BAc, BMp, BUf, and a common B. aphidicola strain associated with Acyrthosiphon pisum (BAp). In view of the obligate symbiosis between B. aphidicola and its host, the RFLP pattern of the yba3-4 region may be a new and useful marker for identifying aphids belonging to different genera. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。