查詢結果分析
相關文獻
頁籤選單縮合
題名 | Chronic and Repeated Chlamydophila Pneumoniae Lung Infection can Result in Increasing IL-4 Gene Expression and Thickness of Airway Subepithelial Basement Membrane in Mice |
---|---|
作者 | Chen, Chiung-zuei; Yang, Bei-chang; Lin, Tsun-mei; Lee, Cheng-hung; Hsiue, Tzuen-ren; | 書刊名 | Journal of the Formosan Medical Association |
卷期 | 108:1 2009.01[民98.01] |
頁次 | 頁45-52 |
分類號 | 415.4 |
關鍵詞 | Airway remodeling; Chlamydophila pneumoniae; Interleukin-4; |
語文 | 英文(English) |
英文摘要 | Background/Purpose: Chlamydophila pneumoniae infection has been associated with several pulmonary and cardiac diseases. However, it has not been explored for its ability to activate the same immunopathologic mechanisms of asthma, namely, a predominant Th2 immune response and structural changes that are termed airway remodeling. This study evaluated immune responses in the lung and airway pathology of BALB/c mice with chronic and repeated C. pneumoniae infections. Methods: Mice were inoculated intranasally with 5 x 106 inclusion-forming units of C. pneumoniae TWAR strain, and re-inoculated at 14 and 42 days after the primary inoculation. Cytokine gene expression in bronchoalveolar lavage (BAL) cells was analyzed by RT-PCR on day 70. Airway pathology was also evaluated by morphometric measurements. Results: A significant increase of interleukin (IL)-4 mRNA was detected in BAL cells in infected mice, and a significant increase in subepithelial basement membrane thickness of the airways was also noted in infected mice as compared with control mice (8.95 ± 0.28 μm vs. 5.54 ± 0.22 μm, p < 0.0001). We further analyzed the correlation between IL-4 cytokine expression and the increased subepithelial basement membrane thickness of airways in infected mice. We found that mice with increased IL-4 mRNA expression had significant increases in the thickness of subepithelial basement membrane as compared with mice without increased IL-4 mRNA expression (9.87 ± 0.51 μm vs. 6.49 ± 0.52 μm, p < 0.0001). Conclusion: It is believed that our results demonstrated for the first time that chronic and repeated infections with C. pneumoniae increased IL-4 gene expression and thickness of airway subepithelial basement membrane in mice. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。