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頁籤選單縮合
題 名 | Karyotyping of Brassica Oleracea L. Based on Cot-1 and Ribosomal DNAs=甘藍基於Cot-1 DNA及rDNA的核型分析 |
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作 者 | 魏文輝; 張蘇鋒; 王力軍; 陳波; 伍曉明; 宋運淳; | 書刊名 | Botanical Studies |
卷 期 | 48:3 2007.07[民96.07] |
頁 次 | 頁255-261 |
分類號 | 435.233 |
關鍵詞 | 核型分析; 甘藍; Cot-1 DNA; rDNA; Brassica oleracea L.; Karyotyping; Ribosomal DNA; |
語 文 | 英文(English) |
中文摘要 | 為了探索一種簡單、可靠、有效的甘藍核型分析新方法,我們用rDNA結合Cot-1 rDNA作為甘藍染色體識別標記,成功地對甘藍進行了準確的核型分析。甘藍基因組DNA用生物素切刻平移試劑盒標記後作探針,原位雜交到有絲分裂中期相,每對染色體均顯示出特定的螢光帶型。25S及5S rDNAs分別用毛地黃素及生物素切刻平移試劑盒標記後作探針,各自原位雜交到有絲分裂中期相,有2對染色體檢測出25S rDNA雜交信號,只有1對染色體檢測出較弱的5S rDNA雜交信號。Cot-1 DNA包含rDNA,雙色螢光原位雜交顯示,25S rDNA與Cot-1 DNA雜交信號位置是一致的,表明基於rDNA及Cot-1 DNA的核型分析技術優於單獨使用一種標記技術。基於rDNA位置及Cot-1 DNA螢光帶,本研究對甘藍進行了精確的核型分析。 |
英文摘要 | To explore a simple, reliable, and effective method of karyotyping Brassica oleracea L., Cot-1 DNA was isolated from its genome, labeled as probe with a Biotin-ick Translation Mix kit, and in situ hybridized to mitotic spreads. Specific fluorescent bands appeared on each chromosome pair. 25S and 5S rDNAs were labeled as probes with a DIG-Nick Translation Mix ket and Biotin-Nick Translation Mix kit, respectively, and in situ hybridized to mitotic preparations. Signals could be detected on two chromosome pairs for 25S rDNA, and on only one for 5S rDNA. Cot-1 DNA contains rDNA. The site identity of Cot-1 DNA and 25S rDNA on the chromosome was determined by dual-colour fluorescence in situ hybridization (FISH). It showed that the karyotyping technique based on a combination of rDNA and Cot-1 DNA chromosome markers is a superior alternative. A more exact karyotype of B. oleracea has been developed based on rDNA locations and Cot-1 DNA fluorescent bands. |
本系統中英文摘要資訊取自各篇刊載內容。