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題名 | A Mutant of Bacillus Subtilis Strain LB5 with Enhanced Antifungal Activities Against Colletotrichum Gloeosporioides=增強對檬果炭疽病拮抗力枯草菌突變株之誘發 |
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作 者 | 張誌益; 梁文進; 趙永椿; 何婉清; | 書刊名 | 植物保護學會會刊 |
卷期 | 49:3 2007.09[民96.09] |
頁次 | 頁213-228 |
分類號 | 433.4 |
關鍵詞 | 枯草桿菌; LB5菌株; LB5'突變株; 拮抗細菌; 丁醇; 16S rRNA序列; Colletotrichum gloeosporioides; Bacillus subtilis LB5 and LB5'; N-butanol; Antifungal bioactivity; 16S rRNA gene sequences; Mutation; |
語文 | 英文(English) |
中文摘要 | 枯草桿菌(Bacillus subtilis) LB5菌株是防治 Colletotrichum gloeosporiodies 引起之檬果炭疽病之拮抗細菌。當以丁醇純化粹取其拮抗代謝物時,獲得一具耐丁醇之突變菌株LB5'。比較兩菌株與Bacillus subtilis標準株之生化特性及PCR 增殖之16S rRNA序列,確定為Bacillus subtilis LB5菌株及其LB5'突變株。分析比對LB5菌株及LB5'突變株之16S rRNA序列可區分兩者之差異。LB5'突變株在生長速率及拮抗真菌菌絲生長能力都比LB5菌株強。 |
英文摘要 | The bacterium antagonist Bacillus subtilis strain LB5 was successfully used as biological control agent to control mango anthracnose caused by Colletotrichum gloeosporioides on Jingkwang cultivar at Pingtung County of Taiwan in 2003 and 2004 growing seasons. During the extraction of antifungal metabolites secreted by LB5 using n-butanol, a novel solvent tolerant strain called LB5' was found. The taxonomic position of these bacteria was studied on the basis of 16S rRNA sequence analysis methods. Comparative computer database analysis demonstrated that the two bacteria were Bacillus subtilis strains and the wild type was closer to Bacillus subtilis (BS) reference strain. Alignment of 16S rRNA sequences by BLAST system highlighted the point mutation induced by n-butanol solvent and enabled to differentiate the two strains. The solvent tolerant mutant B. subtilis LB5' had a higher cell density and better antifungal inhibitory effects than the wild type LB5 during their cultivation for metabolites production. |
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