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題名 | 利用種子蛋白電泳及西方墨點分析進行大豆屬物種分群之研究=Using Seed Protein Electrophoresis Profile and Western Blotting to Analyze the Classification of Glycine Species |
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作者 | 蔡元卿; 謝宗涵; 陳映伶; 彭雅涓; 邢禹依; 謝兆樞; | 書刊名 | 作物、環境與生物資訊 |
卷期 | 3:2 民95.06 |
頁次 | 頁159-176 |
分類號 | 434.215 |
關鍵詞 | 大豆屬物種; 聚丙烯醯膠片電泳; 大豆種子成熟蛋白; 西方墨點轉漬法; Glycine species; Ployacrylamide gel electrophresis; Soybean seed maturation protein; Western blot; |
語文 | 中文(Chinese) |
中文摘要 | 摘要 野生大豆是栽培種大豆在實行抗病抗蟲 耐旱等育種改良計畫時重要的基因庫,不同 的野生種對於有益性狀有不同的貢獻,快速 鑑別大豆屬物種有助於育種親本的選定,加 速整個育種計畫流程。在許多試驗中證明種 子蛋白電泳可以進行物種鑑定,搭配種子蛋 白抗體進行西方墨點轉漬法分析,更是一項 進行辨認大豆屬物種的有利工具。多種大豆 屬物種的種子全蛋白以SDS-PAGE 與西方墨 點轉漬法進行分析,結果顯示每一個物種均 有其獨特的電泳圖譜,可以做為物種鑑識之 用。本研究用於免疫分析共有九個種子蛋 白,其中變異少的蛋白有MP130、glycinin、 GmPM4、GmPM5、GmPM16 、GmPM28 等六個種子儲存蛋白或成熟蛋白;而變異多 的蛋白有GmPM1、GmPM2、GmPM8 等三 個種子成熟蛋白。依據這些種子蛋白的分析 結果,針對G. tomentella 物種複合群所做的 分群與前人利用同功群的方法所推論出的 結果一致。 |
英文摘要 | ABSTRACT The Glycine species are the important gene pools for improvement of soybean crop against insect, disease and drought stress, comparing to the narrower germplasm of the cutivar soybean. Identifying useful trait doners as parents for breeding effectively can facilitate the breeding program. Many experiments had confirmed that seed proteins electrophoresis can be used as a tool for plant taxonomy, and the efficiency will increase if collocate with Western blotting. In the present study, the seed proteins from several Glycine species were analyzed by sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE) and Western blot against soybean seed maturation protein and seed storage protein antibodies. Eachspecies yields a unique electrophoretic pattern that varied in the total number of bands and their relative mobilities. Thus, we may use these protein profiles as a tool to identify Glycine species. Six seed protein products, including MP130, Glycinin, GmPM4, GmPM5, GmPM16, and GmPM28, are less varied, and the other three proteins, GmPM1, GmPM2 and Gm PM8, are more varied among Glycine species. The results thus gave a similar conclusion as those provided by isozyme studies described previously by other group. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。