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題名 | 龍膽石斑稚魚之飼料維生素E需求及其免疫反應=Dietary Vitamin E Requirement of Giant Grouper, Epinephelus Lanceolatus, and Its Effects on Immune Responses |
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作 者 | 吳豐成; 葉信利; | 書刊名 | 水產研究 |
卷期 | 16:1 2008.06[民97.06] |
頁次 | 頁77-85 |
分類號 | 439.5 |
關鍵詞 | 龍膽石斑; 維生素E需求; 免疫反應; Giant grouper; Epinephelus lanceolatus; Vitamin E requirement; Immune response; |
語文 | 中文(Chinese) |
中文摘要 | 本研究以添加七種不同濃度 (0、7.5、15、30、60、120和 240 mg/kg) 的維生素E (all-rac-α-tocopherol) 飼料,餵飼平均體重8.5 ± 0.5 g的龍膽石斑 (Epinephelus lanceolatus) 稚魚12週,以探討其對維生素E之需求,以及維生素E對其頭腎白血球的吞噬、呼吸爆 (respiratory burst) 等活性和白血球增生之影響。結果顯示,飼料中添加維生素E顯著 (p < 0.05) 影響龍膽石斑之成長、活存率和肌肉硫巴比妥酸值 (TBARS)。攝取添加維生素E大於或等於15 mg/kg DW飼料組之試驗魚的頭腎白血球吞噬活性及增生作用顯著高於未添加組,但對白血球的呼吸爆活性則無影響。將成長結果以Broken-line模式分析後,可得出龍膽石斑魚的維生素E需求量為46 mg/kg DW (95% 信賴區間為32.1 ~ 59.9 mg/kg DW)。 |
英文摘要 | Juvenile giant grouper (Epinephelus lanceolatus) were fed experimental diets with one of seven levels of vitamin E (all-rac-α-tocopherol) (0, 7.5, 15, 30, 60, 120 and 240 mg/kg diet) for 12 weeks to investigate the dietary requirement and their effects on phagocytic, respiratory burst and leukocyte proliferative activities. Significant differences (p < 0.05) in growth, survival rate and muscle TBARS (thiobarbituric acid-reactive substance) were observed among the dietary treatments. Enhanced growth was observed when the diets supplemented with vitamin E, indicating that the vitamin E was requirement for grouper. When fish fed diet with vitamin E greater or equal to 15 mg /kg, phagocytic and proliferative activities of head-kidney leukocytes were significantly enhanced. However, the supplemented vitamin E did not have effects on respiratory burst activity of head-kidney leukocytes. Weight gain analyzed by broken-line model indicated that the optimum vitamin E concentration for juvenile giant group was 46 mg/kg in diet with lower and upper 95% confidence levels on 32.1 and 59.9 mg/kg, respectively. |
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