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題名 | 銅、鐵離子及維生素B群對Alexandrium Minutum生長與產毒之影響=The Effects of Cu, Fe and Vitamin B Group on the Growth and Toxicity of Alexandrium Minutum |
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作者 | 張淑貞; 黃秋菊; 陳堯鈴; 吳天祚; 賴文亮; 蔡永祥; Chang, Shu-chen; Hwang, Chiu-chu; Chen, Yao-ling; Wu, Tien-tso; Lai, Wen-liang; Tsai, Yung-hsiang; |
期刊 | 大仁學報 |
出版日期 | 20061200 |
卷期 | 29 民95.12 |
頁次 | 頁13-30 |
分類號 | 418.8 |
語文 | chi |
關鍵詞 | 微量元素; 小亞歷山大藻; 麻痺性貝毒; 毒性; Trace elements; Alexandrium minutum; Paralytic shellfish poison; Toxicity; |
中文摘要 | 為了解水域中之銅、鐵離子及維生素B群是否會影響毒藻A. minutum之生長與產毒,本實驗即以不同氧化態及不同濃度之銅離子、鐵離子以及維生素B群於walne medium配置時分別加入以培養藻類,結果發現毒藻A. minutum之最適生長與產毒條件以walne medium之條件最佳,即銅離子(Cu²⁺)濃度5 ppb、鐵離子(Fe³⁺) 濃度270 ppb以及維生素B₁ 200 ppb與維生素B[feba] 10 ppb。若利用亞銅離子 (Cu²)及亞鐵離子(Fe²⁺)取代walne medium中之銅離子及鐵離子之含量,結果發現生長與產毒均不佳,顯示其無法取代原本培養液中Cu²⁺、Fe³⁺之使用。以(HPLC分析藻體毒素之組成種類皆為GTX□為主,且以GTX₁和GTX₄佔多數。 |
英文摘要 | Paralytic shellfish poisoning (PSP) associated with food poisoning incidents have caused two human fatalities and many illnesses in Taiwan. The sourse of PSP in Taiwan was thought to be the toxic dinoflagellate alexandrium minutum. To understand the Cu. Fe and vitamin B group in walne medium for cell growth and toxin production of A. minutum, the different concentration of copper and iron ion, and vitamin B group were added into the walne medium of A. minutum. It was found that the optimal trace elements for those of A. minutum were as follows: copper ion (Cu²⁺) 5.0 ppb, ferric ion (Fe³⁺) 270 ppb, and vitamine B₁ 200 ppb and vitamin B□ 10 ppb. The toxic components of A. minutum were assayed by high performance liquid chromatography (HPLC), and found to contain gonyautoxin (GTX)□ only. Among these components, GTX₁ and GTX₄ were predominate components. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。