頁籤選單縮合
題名 | Suppression of Tumorigenicity of Human Hepatocellular Carcinoma Cells by Antisense Oligonucleotide MZF-1 |
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作者 | Hsieh, Yi-hsien; Wu, Trang-tiau; Huang, Chih-yang; Hsieh, Yih-shou; Liu, Jer-yuh; | 書刊名 | 中國生理學雜誌 |
卷期 | 50:1 2007.02[民96.02] |
頁次 | 頁9-15 |
分類號 | 397 |
關鍵詞 | Myeloid zinc finger-1; Tumorigenesis; Antisense oligonucleotide; |
語文 | 英文(English) |
英文摘要 | Our previous studies have found that reducing expression of myeloid zinc finger-1 (MZF-1) inhibited protein kinase C alpha (PKCα) expression and decreased cell migration and invasion in human hepatocellular carcinoma (HCC). In this study, we further investigated the role of MZF-1 in tumorigensis. The SK-HEP-1 HCC cells were transected with the antisense oligonucleotide (ODN) of MZF-1. The pretreated cells was then detected the mRNA and protein levels by RT-PCR and western blotting, and the cell growth was assayed by MTT. Meanwhile, the pretreated-SK-Hep-1 HCC cells were implanted subcutaneously into nude mice to observe the tumor growth and calculated tumor inhibitory rate. The results showed that, at the dosage of 5μM, the antisense ODN MZF-1 suppressed both MZF-1 and PKCα productions by SK-Hep-1 Hcc cells after cationic liposome-mediated transfection, to 15% and 30% in control cultures of 0μM dosage, respectively. The growth of SK-Hep-1 HCC cells was inhibited by the 2-5μM doses of the antisense ODN MZF-1, whereas the control reagent, the sense ODN MZF-1, showed no effects. In BALB/nude mice, SK-Hep-1 HCC cells pretreated with the 5μM antisense ODN MZF-1 formed tumors much smaller than the cells with sense ODN. The mean of inhibitory rate of tumor growth was 71.2±8.6%, and the tumor formation time was prolonged from 14 days to 26 days. These findings suggested the usefulness of antisense ODN MZF-1 as a new reagent for cancer therapy. |
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