頁籤選單縮合
題名 | Optimal Hepatitis B Virus Primer Sequence Design for Isothermal Amplification= |
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作者 | Lee, Szu-yuan; Lee, Chun-nan; Mark, Holl; Meldrum, Deirdre R.; Lee, Chih-kung; Lin, Chii-wann; |
期刊 | Biomedical Engineering: Applications, Basis and Communications |
出版日期 | 20070600 |
卷期 | 19:3 2007.06[民96.06] |
頁次 | 頁137-144 |
分類號 | 410.1644 |
語文 | eng |
關鍵詞 | Hepatitis B virus; LAMP; Isothermal; Primer design; |
英文摘要 | We have reported the successful use of the Loop Mediated isothermal amplification (LAMP) reaction for Hepatitis B Virus (HBV) DNA in a 25N<l micro-reactor for the development of a rapid diagnostic device recently. This method shows the successful amplification of HBV DNA with high specificity and sensitivity efficiently within one hour. Such a powerful method can be used for many other fast diagnostic applications with the help of optimal primer design and miniaturization of reactor. In this report, we will first summarize the general design rules for primer design from literature reviews and our experimental results. We will discuss the effects of HBV primers and concentrations of working buffers for optimal performance. |
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