頁籤選單縮合
題名 | Apoptotic Effects of Over-Expressed Estrogen Receptor-β on Lo Vo Colon Cancer Cell is Mediated by p53 Signalings in a Ligand-Dependent Manner |
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作者姓名(外文) | Hsu, Hsi-hsien; Cheng, Sue-fei; Wu, Cheng-chung; Chu, Chun-hsien; Weng, Yi-jiun; Lin, Chung-sheng; Lee, Shin-da; Wu, Hung-chien; Huang, Chih-yang; Kuo, Wei-wen; | 書刊名 | 中國生理學雜誌 |
卷期 | 49:2 民95.04 |
頁次 | 頁110-116 |
分類號 | 416.245 |
關鍵詞 | Estrogen receptor-β; Lo Vo cells; Apoptotic effects; Antiproliferation; |
語文 | 英文(English) |
英文摘要 | Epidemiologic studies reported that the prevalence of hereditary non-polyposis colon cancer (HNPCC) in the male is about 1.5-fold higher than that in female. Decreases in circulatory estradiol (□) have been reported to downregulate the expression of □ receptor (ER) and significantly increase the risk of colorexctal cancer. Patients that received □ replacement therapy were found to have a reduction in the incidence of colon adenoma and carcinoma. Furthermore, significant decreases in the expression of □and ER against colorectal cancer. However, the mechanisms remain unexplored. LoVo cells were transient transfected to overexpress ER-β, DNA fragmentation and caspase activity assay were performed to evaluate apoptotic effects. Western blotting was used to evaluate activity assay were performed to evaluate apoptotic effects. Western blotting was used to evaluate protein levels, and inciferase activity assay to measure the TNF-α promoter activity. Our data clearly demonstrated that □ and ER-β did not influence the expression and promoter activity of TNF-α. In addition, □ and overexpressed ER-β did not influence the expression and promoter activity of TNF-α. In addition, □ and overexpressed ER-β downregulated the β-catenin proteins which cause the downregulation of its target genes, cycling D1 and Rb. to inhibit the cell cycle and cell proliferation. The results indicate that overexpressied ER-β may induce LoVo cell apoptosis and anti-proliferation by increasing p53 signaling in a ligand-dependent manner, and without TNF-α involvement. Efforts aiming at enhancing ER-β expression and/or activity may prove to be an attractive alternative therapy against colorectal cancer. |
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