查詢結果分析
相關文獻
- Evaluating the Validity of the Serologic Test for Detecting Helicobacter Pylori Infection in Mongolian Gerbils
- Immunoglobulin G Antibody Against Helicobacter pylori Is an Accurate Test for Atrophic Gastritis
- 漫談幽門螺旋桿菌感染及基層醫療之對策
- New One-Week, Low-Dose Triple Therapy for the Treatment of Duodenal Ulcer with Helicobacter Pylori Infection
- A Multicenter Study on Eradication of Helicobacter pylori Infection in Patients with Duodenal Ulcer by Lansoprazole-Antibiotics Combined Therapy
- 消化性潰瘍的元兇--幽門螺旋桿菌
- 大黃抑制幽門螺旋桿菌及其作用機轉之研究
- 醇提大黃對消化性潰瘍合併幽門螺旋桿菌感染之作用探討
- 胃幽門螺旋桿菌的微生物學與流行病學
- 胃幽門螺旋桿菌與消化性疾病之因果關係
頁籤選單縮合
題名 | Evaluating the Validity of the Serologic Test for Detecting Helicobacter Pylori Infection in Mongolian Gerbils=評估血清學檢測法在蒙古沙鼠感染幽門螺旋桿菌模式之正確性 |
---|---|
作者姓名(中文) | 郭昭宏; 余方榮; 蔡珮芸; 楊曉芳; 張玲麗; 詹昌明; 王文明; 吳登強; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷期 | 23:11 2007.11[民96.11] |
頁次 | 頁545-551 |
分類號 | 414.83 |
關鍵詞 | 幽門螺旋桿菌; 蒙古沙鼠; 血清學檢查; Helicobacter pylori; Mongolian gerbil; Serologic test; |
語文 | 英文(English) |
中文摘要 | 幽門螺旋桿菌 (Helicobacter pylori) 和胃癌之相關性已被許多研究證實。近來,蒙古沙鼠已被認為是研究幽門螺旋桿菌導致胃癌的最佳動物模式。本研究之目的在於評估血清學檢測方式用在蒙古沙鼠模式之正確性及效益。我們使用 50 隻 8 週大之雄性沙鼠,並以之前沙鼠黏膜培養出的 (VacA(+)/CagA(+)) 之幽門螺旋桿菌菌株餵食,幽門桿菌之餵食濃度為 109/CFU,以胃管餵食 (IG) 之方式餵食上述溶液 0.5-1.0 mL,餵食之頻率為 2 次,間隔 3 天。餵食幽門桿菌後,分別在第 6 週及第 26 週分別各犧牲 25 隻沙鼠。在犧牲前由沙鼠尾部取得血液進行 STAT-PAK 測驗,犧牲後,取得沙鼠胃前壁黏膜進行下列測試:培養、組織學、快速尿素酶法 (RUT) 及 PCR,以確認沙鼠感染幽門桿菌與否。再以此結果評估 STAT-PAK 方法之正確性。在 50 隻之餵食幽門桿菌的沙鼠中,成功感染率為 44/50 (88%),而對照組之 10 隻沙鼠均呈陰性反應。我們發現沙鼠胃壁之黏膜受損隨著餵食期間的增加更明顯。STAT-PAK 方法的敏感度:85.6%,特異度 100%,陽性預測值 100%,陰性預測值 50%,正確率為 88%,且幽門桿菌感染密度較高時,STAT-PAK 之正確率較高。我們成功地建立一個穩定且長期之蒙古沙鼠感染幽門桿菌之模型,研究顯示 STAT-PAK 法 (血清學法) 呈現可接受之結果,且非常適合於蒙古沙鼠模式之長期觀察上。 |
英文摘要 | A strong correlation between Helicobacter pylori infection and gastric cancer has been reported. Mongolian gerbils are regarded as the most suitable animal model in which to study carcinogenesis associated with H. pylori. The aim of our study was to evaluate the accuracy of the serologic test for detecting H. pylori infection in Mongolian gerbils. The model was developed as follows: the H. pylori colony (vacuolating cytotoxin A (+)/cytotoxin-associated gene A (+)) was cultured from the mucosas of previously H. pylori-fed gerbils. These colonies were cultured in broth. Then, we fed the gerbils with 0.51 mL of broth (about 109 CFU/mL) (intragastric administration) twice within a 3-day period. After inoculation for 6 or 26 weeks, the gerbils were sacrificed and their gastric mucosas were sampled for a series of examinations. Blood samples for serologic testing (STAT-PAK) were collected. H. pylori infection was confirmed. Statistical analysis was performed using the χ2 test. Differences were regarded as significant when the p value was less than 0.05. A total of 50 gerbils were inoculated with H. pylori and the success rate reached 88%. All 10 gerbils in the control group showed a negative result. Damage to the mucosas was more obvious following increasing periods of inoculation. The rates of sensitivity and specificity, as determined by the STAT-PAK test, were 90.9% and 100%, respectively. The positive and negative predictive values were 100% and 60%, respectively. The STAT-PAK test seemed to be more sensitive and accurate (p < 0.05) in high H. pylori densities. In conclusion, the STAT-PAK test (blood-sampling) showed acceptable results and was suitable for long-term observation of H. pylori infection. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。