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題 名 | Use of Caveolin-1, Thyroid Transcription Factor-1, and Cytokeratins 7 and 20 in Discriminating between Primary and Secondary Pulmonary Adenocarcinoma from Breast or Colonic Origin=利用Caveolin-1,TTF-1,CK7和CK20來區分原發性肺腺癌和來自乳房或大腸的轉移性肺腺癌 |
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作 者 | 曹淑娟; 蘇月秋; 王勝嵐; 蔡志仁; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 23:7 2007.07[民96.07] |
頁 次 | 頁325-331 |
分類號 | 415.468 |
關鍵詞 | 肺腺癌; CK7; CK20; TTF-1; Caveolin-1; Cytokeratin 7; Cytokeratin 20; Pulmonary adenocarcinoma; Thyroid transcription factor-1; |
語 文 | 英文(English) |
中文摘要 | 本篇研究的目的首先是要比較 caveolin-1、TTF-1、CK7 和 CK20 個別在原發性肺腺癌和來自乳房或大腸的轉移性肺腺癌的表現並且調查這些因子單獨使用或是聯合這些因子的表現比較能區分原發性肺腺癌和來自乳房或大腸的轉移性肺腺癌。此次研究所選定的 49 個肺腺癌樣本,分別為 30 個原發性肺腺癌和 19 個轉移性肺腺癌 (包括有 9 個來自乳房轉移和 10 個來自大腸轉移之肺腺癌樣本)。我們利用免疫組織化學染色的方法來評估 caveolin-1、TTF-1、CK7 和 CK20 的表現。當染色結果呈現 CK7 陽性/CK20 陰性並且 TTF-1 為陽性或 caveolin-1 陰性時最有可能的是原發性肺腺癌。當呈現 CK7 陽性/CK20 陰性及 TTF-1 陰性或 caveolin-1 陽性最可能是來自乳房轉移的肺腺癌。若染色結果為 CK20 陽性/CK7 陰性和 TTF-1 陰性或 caveolin-1 陽性則最可能是來自大腸轉移的肺腺癌。本篇研究結果顯示,單獨使用 caveolin-1、TTF-1、或 CK7/CK20 並不能有效的區別原發性肺腺癌和來自乳房或大腸的轉移性肺腺癌。聯合這些因子時敏感性較高比較能區分肺腺癌是原發性的或是從乳房、大腸轉移來的。 |
英文摘要 | The objectives of this study were firstly to compare the immunostaining patterns of antibodies against caveolin-1, thyroid transcription factor-1 (TTF-1), cytokeratin 7 (CK7) and cytokeratin 20 (CK20) in primary and secondary pulmonary adenocarcinomas of breast or colonic origin, and secondly, to investigate their use alone and in combination, in distinguishing between primary and secondary lung adenocarcinomas from breast or colonic origin. Of the 49 lung adenocarcinoma specimens that were enrolled in this study, 30 were primary pulmonary adenocarcinomas, and 19 (9, breast origin; 10, colonic origin) were metastatic pulmonary carcinomas. Immunohistochemical staining was used to detect the expression of caveolin-1, TTF-1, CK7, and CK20. Primary pulmonary adenocarcinoma most often had the CK7-positive/CK20-negative immunohistochemical phenotype and was either TTF-1 positive or caveolin-1 negative. Secondary pulmonary adenocarcinoma of breast origin most often had the CK7-positive/CK20-negative immunohistochemical phenotype and was either TTF-1 negative or caveolin-1 positive, while secondary pulmonary adenocarcinoma of colonic origin most often had the CK20-positive/CK7-negativeimmunohistochemical phenotype and was either TTF-1 negative or caveolin-1 positive. The results suggest that caveolin-1, TTF-1, or CK7/CK20 alone did not distinguish reliably between primary and secondary pulmonary adenocarcinomas originating from breast or colon. The use of a panel of antibodies that includes TTF-1, caveolin-1, and CK7/CK20 may have higher sensitivity in discriminating between primary adenocarcinomas and metastatic lung adenocarcinomas from breast or colonic origin. |
本系統中英文摘要資訊取自各篇刊載內容。