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題 名 | 橫山梨及鳥梨莖頂組織培養之研究 |
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作 者 | 廖萬正; 王芳美; 楊耀祥; | 書刊名 | 臺中區農業改良場研究彙報 |
卷 期 | 34 1992.03[民81.03] |
頁 次 | 頁1-9 |
分類號 | 435.312 |
關鍵詞 | 組織培養; 莖頂; 鳥梨; 橫山梨; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究係利用組織培養之技術,以橫山梨(Pyrus serotina Rehd. cv . Hengshan)及鳥梨(Pyrus kawakamii Hay. cv. Niauli)之莖頂加以培養,調查影響其 成活之因子及探討各培植時期最適當之培養基及培養方法,以建立大量迅速之繁 殖技術。在橫山梨及鳥梨新梢伸長旺盛期,採取長約5cm頂部,於解剖顯微鏡 下切取長約0.2∼0.3mm含1∼2個葉原體之莖頂,植入MS、1/2MS、1/4MS、 1/8MS等4種不同濃度之固態及濾紙液態培養基中,經30日培養後,結果以 1/2MS濾紙培養基效果最佳。增殖培養基則以MS為基本培養基添加2mg/l BA、 0.2mg/l IBA、0.5mg/l kinetin、4 mg/l adenine sulfate、30g/l sucrose及8g/l agar最 佳。每30日可繼代培養一次,橫山梨及鳥梨每芽體可分別獲得5.8支及9.7支長 約5mm左右可供再增殖用之枝梢。再經30日之培養,橫山梨每芽體可得4.3支, 鳥梨可得7.8支長約30mm可供為誘導發根之枝梢。橫山梨枝梢扦插於1/2MS 添加162mg/l phloro-glucinol (PG)及0.1mg/l IBA或1mg/l IBA之培養基,經暗處 理6日後,再移入不含auxin之1/2MS培養基,約30日後可獲得60%已發根之 小苗。鳥梨扦插於1/2MS添加162mg/l PG及1mg/l IBA或PG及0.1mg/l NAA 或PG及1mg/l NAA培養基中,經6日之暗處理後,移入不含auxin之1/2MS培 養基中,再經30日培養後可獲得約70%以上之已發根小苗。 |
英文摘要 | The effects of culture media and methods on the mass propagation and survival rate of shoot tips of Pyrus serotina Rehd. cv. Hengshan and Pyrus kawakamii Hay. cv. Niauli in vitro were studied. Young shoot tips about 5 cm in length were harvested from actively field-grown plants in March. The shoot tips were dissected and apical portions (0.2-0.3mm) were removed under stereo microscope, of which containing 1-2leaf primodia. These explants were cultured on the paper bridge media containing half-strength Murashige and Skoog salt (1/2MS), 0.2mg/l IBA, 1mg/l BA, 0.5mg/l kinetin, 4mg/l adenine sulfate and 30g/l sucrose, for 30 days. They were transferred to solid media (same component as above mentioned media) for another 45 days. The explant was then transplanted to MS medium containing 0.2mg/l IBA, 2mg/l BA, 0.5mg/l kinetin, 4mg/l adenine sulfate, 30g/l sucrose and 8g/l agar for shoot multiplication. Each subculture can harvested 5mm in length of 5.8 and 9.7 multipliable shoots for Hengshan and Niauli within 30 days, respectively. If subcultured for 60 days, it can harvested 4.3 and 7.8 shoots of 30mm in length for Hengshan and Niauli, respectively, and was ready for root induction. The shoots of Hengshan pear were placed on 1/2 MS medium containing phloroglucinol (PG) plus 0.1mg/l IBA or 1mg/l IBA and kept in the dark for 6 days, then transplanted to auxin-free 1/2 MS medium, 60% of rooted plantlets were obtained after 30 days. The shoots of Niauli were cultued on 1/2 MS medium with 162mg/l PG plus 1mg/l IBA, or 0.1mg/l NAA or 1mg/l NAA and kept in the dark for 6 days, then transferred to auxin-free 1/2 MS medium, 70-100% of rooted plantlets were obtained after 30 days. |
本系統中英文摘要資訊取自各篇刊載內容。