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題名 | Biohydrogen Production Using Suspended and Immobilized Mixed Microflora=以懸浮與固定化混合菌群進行生物醱酵產氫 |
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作者姓名(中文) | 吳坤哲; 張嘉修; 張瓊妃; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷期 | 37:6 民95.11 |
頁次 | 頁545-550 |
分類號 | 368.7 |
關鍵詞 | 懸浮細胞; 固定化厭氧污泥; 醱酵產氫; Biohydrogen production; Immobilized cell; Alginate; Composite material; Anaerobic sludge; |
語文 | 英文(English) |
中文摘要 | 本研究利用蔗糖為碳源,以懸浮和固定化厭氧污泥進行批次醱酵產氫試驗。藉由一系列的固定化方法來測試產氫材料的效率與穩定性。結果顯示以複合材料(藻酸鈣+氧化鈦+幾丁聚醣)製備之固定化細胞產氫效果最佳,於35℃和蔗糖濃度為20 g COD•L¯¹時,可得產氫速率為21.3 mmol•L¯¹•h¯¹,此速率為懸浮細胞(6.8 mmol•L¯¹•h¯¹)的三倍多。在此批次產氫試驗中,蔗糖轉化率可達85%以上,氣相產物只含氫氣和二氧化碳,主要的液相代謝產物為丁酸、乙酸、丙酸和少量的乙醇。於懸浮細胞批次試驗中,添加微量的Ca²+和Ni²+離子,可讓產氫速率從6.8 mmol•L¯¹•h¯¹分別增至9.3 mmol•L¯¹•h¯¹和8.7 mmol•L¯¹•h¯¹。此外,本研究結果亦發現,添加氧化鈦之共固定化細胞材質可有效地增進生物產氫效率。 |
英文摘要 | Suspended and immobilized anaerobic sludge were utilized to produce hydrogen gas in batch reactors using sucrose as the sole carbon substrate. A variety of immobilization matrices and methods were examined for their suitability to make immobilized H₂ producers. The results show that cells immobilized by calcium alginate matrix supplemented with chitosan and titanium oxide carriers (CA+CH+TiO2) were the most effective in H₂ production, giving a H₂ production rate of 21.3 mmol•L¯¹•h¯¹ (at 35℃ and 20 g COD•L¯¹ of sucrose), which was over three-fold of that obtained from suspended cells (6.8 mmol•L¯¹•h¯¹). The sucrose conversion was essentially 85% or higher in all runs, and the biogas consisted of only H₂ and CO₂. The major soluble metabolites were butyric acid, acetic acid, and propionic acid, while a small amount of ethanol was also produced. Addition of trace metal salts (Ca²+ and Ni²+) appeared to enhance the H₂ producing performance for the suspended culture, elevating H₂ production rate from 6.8 to 9.3 and 8.7 mmol•L¯¹•h¯¹, respectively. Moreover, the immobilization matrix containing TiO₂ seemed to result in better H₂ production in general. |
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