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題名 | 酵素法合成右旋之胺基酸與多肽=Enzymatic Synthesis of D-amino Acids and their Oligomers |
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作者姓名(中文) | 戴達夫; 葉艾璿; | 書刊名 | 化學 |
卷期 | 64:3 民95.09 |
頁次 | 頁377-386 |
分類號 | 346.01 |
關鍵詞 | 右旋胺基酸; 酵素合成法; 含右旋胺基酸之胜肽; 胜肽合成; D-amino acids; Enzymatic synthesis; Peptides containing D-amino acids; Peptide synthesis; |
語文 | 中文(Chinese) |
中文摘要 | 一些以酵素催化生產右旋胺基酸和含右旋胺基酸胜已被發展出來。目前具光學純度之右旋胺基酸的工業上生產方法,乃是使用對右旋胺基酸或左旋胺基酸專一的酵素,將醯化之消旋胺基酸、消旋胺基酸醯胺,進行生物轉化。類似的,右旋胺基酸也可由對應之左旋胺基酸產生,先轉變其為消旋胺基酸,然後將消旋的混合物選擇性的轉化,經由於連續的酵素催化反應,高效的生產右旋胺基酸。 右旋胺基酸已經被發現在多種自然的胜中。這些包括鴉片胜和抗菌胜、神經胜、荷爾蒙等。應用部分或者全部由右旋胺基酸組合的胜已重新的引發熱潮。這些胜比自然之胜更加穩定於蛋白酵素之水解,而且他們有潛能成為經由合成產生之具生物活性的成份。 天然的酵素如右旋胺基胜酵素、右旋胺基酸醯胺酵素、右旋胺基酸醯化酵素、右旋乙醯内酵素、蛋白水解酵素或脂肪水解酵素,能被應用來製造右旋胺基酸,或在適當的反應條件下促進逆反應,來合成右旋胺基酸多。 最後,依據右旋胺基酸之衍生物是否能成為基質以及其被水解的速率,衡量其是否為各別酵素所能接受的醯基提供者、與親核基,這些資訊方便吾等歸納設計出有效率之右旋胜的合成。 |
英文摘要 | Several enzymatic methods for the production of D-amino acids and oligopeptides containing D-amino acids have been developed. Nowadays, optically pure D-amino acids are industrially manufactured by biotransformation of acyl-DL-amino acids, DL-amino acid amides, with a D-isomer or L-isomer specific enzyme. Similarly, D-amino acids have been produced from their corresponding L-isomer, which is transformed to the DL-form. Then the racemic mixture is selectively converted. As a result of successive enzymatic reactions, D-amino acids can be efficiently produced. D-Amino acids have been detected in a variety of natural peptide. These include opiate and antimicrobial peptides, neuropeptides, and hormones. There has been a regain of interest in the applications of peptides assembled partly or totally from D-amino acids. Such peptides are much more stable to proteolysis than natural L-peptides and they have considerable potential as synthetic biologically-active components. Enzymes from nature, such as D-aminopeptidase, D-amino acid amidase, alkaline D-peptidase, D-aminoacylase, proteases, or lipase, either offer potential application in the production of D-amino acids, or the synthesis of their oligomers by promoting the reversed reaction under appropriate conditions. Finally, according to their ability to perform as a substrate and the rate of hydrolysis, D-amino acid derivatives were also evaluated as the specific acyl donor and nucleophile to the individual enzyme. This information facilitates us to design and synthesize D-peptides efficiently. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。