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頁籤選單縮合
題 名 | 夜來香病毒之研究與檢定技術發展現況=Current Status of Tuberose Virus Research and the Development of Its Indexing Techniques |
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作 者 | 張清安; 陳金枝; | 書刊名 | 植物病理學會刊 |
卷 期 | 8:3 1999.09[民88.09] |
頁 次 | 頁89-94 |
分類號 | 435.459 |
關鍵詞 | 夜來香病毒; 夜來香; 晚香玉; Polianthes tuberosa L.; |
語 文 | 中文(Chinese) |
中文摘要 | 夜來香, 又名晚香玉,雖原產於墨西哥,但頗能適應台灣之風土氣候,被視為 具發展潛力之近本土化球根花卉。文獻上有關夜來香病毒病之記載甚少,過去僅紐西蘭曾報 導有馬鈴薯 Y 屬病毒感染之情形,但並未鑑定出確實之病毒種類。 本研究室於 1994 年自 夜來香植株中分離出一種長絲狀病毒,經鑑定後證實為一 Potyviriis 屬之新紀錄病毒。調 查發現本省絕大多數無性繁殖之重瓣品系夜來香均已感染此病毒,由於病株之葉片及花軸上 會出現輕微嵌紋病徵,因此將其命名為夜來香微嵌紋病毒( Tuberosemildmosaicpotyvirus ,簡稱 TMMV )。TMMV 除感染夜來香外並未發現有其他寄主植物。 除機械傷口接觸外其亦 可經由蜿蟲以非永續型方式傳播。以電泳分析估計 TMMV 之稍蛋白分子量約為 38kDa。血清 試驗結果發現 TMMV 與其他 22 種已知之 potyviruses 均不具同源之血緣關係,顯示 TMMV 應為一具獨立分類地位之 Potvirii,屬病毒。 此項結論後經進一步選殖及定序 TMMV RNA 之 3' 端約 2kb 核酸序列並與其他已知之 16 種 potyviruses 之核酸與氨基酸序列比對後 更加確定。 所製備之 TMMV 抗血清可以應用於 ELISA-directtissueblotting ( DTB )及 SDS- immunodittusiontest 等三種方法將 TMMV 由感病植物組織中專一性檢出。 但檢定對 象如果是生長中之值株時三者中以 ELISA 最為適用,若對象為儲藏球根時則以 DTB 最佳, 而 SDS-immunodlitusion test 則較適用於病毒種類之確認。由於發現 TMMV 在夜來香植株 全身部位之濃度分佈頗不均勻,其中以幼嫩新葉及花軸稍葉之病毒濃度較高,故可作為檢定 時之最佳採樣部位,而檢定儲藏期球根時則應以鱗葉為取樣標的,其病毒檢出率之準確度最 高。 試驗中更發現利用 25 ℃回溫處理長期冷藏於 5 ℃之種球,同促使病毒濃度於二天內 迅速提升,增加病毒檢定之正確性。 |
英文摘要 | Tuberose (Polianthes tuberosa L.), a species native to Mexico, has been cultivated in Taiwan for over 300 years and has become an economically important bulb crop. Several diseases including Erwinia spp., Botr@tis spp., and Sclerotium rolfsii have been documented to infect tuberoses, however, there has not been any report on virus disease until 1988 when a virus, possibly a potyvirus, was described in New Zealand. During a survey for ornamental plant virus diseases in 1994, foliar mild mosaic symptoms were noticed on most tuberose plants in Taiwan. A virus was isolated from infected plants and it was subsequently identified as an newly recognized potyvirus, designated as tuberose mild mosaic potyvirus (TMMV) , based on particle morphology, aphid transmissibility and the cylindrical inclusions it induced in infected cells. Tuberose was the only host identified so far for TMMV in our inoculation experiment. The purified capsid contained a single species of protein monomer with an estimated relative mass of 38 kDa. Based on the result of several serological tests, TMMV was found serologically distinct to other 22 known potyviruses tested. This was further confirmed by the comparative sequencing studies of a 2 kb DNA fragment cloned from the 3' terminus of TMMV's RNA. Using the antiserum prepared against purified TMMV virions, we showed that ELISA, tissue blotting and SDS-immunodiffusion test could specifically detect the virus from tuberose tissue. Among them, ELISA was found the most appropriate one for detecting virus infection in leaf tissue, however, tissue blotting was evaluated as the best choice for bulb indexing. TMMV was found distributed unevenly in different parts of tuberose plants. The highest concentration was detected in sheath leaves of flower stems followed by the young leaves; they were reasonably taken as the best sampling sites during field survey. To index tuberose bulbs, however, lateral buds and apex scale leaves were optimum choices for sampling. It was also found that by treating the 4 ℃ -stored bulbs at 25 ℃ for 2 days could enhance virus multiplication in bulb tissue from a minimum to a detectable level thus allowing better and consistent indexing. An antiserum against bacteria expressed cloned TMMV coat protein has been successfully produced and shown that it was applicable, equivalent to the antibodies produced by traditional means, in the detection of TMMV in tuberoses. |
本系統中英文摘要資訊取自各篇刊載內容。