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題 名 | Growth Inhibition and Cell Death Induction by Resveratrol in Human Lung Adenocarcinoma Cells=白蔾蘆醇對肺腺癌細胞生長之抑制作用 |
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作 者 | 詹銘煥; 陳貴評; | 書刊名 | 慈濟醫學 |
卷 期 | 18:5 民95.10 |
頁 次 | 頁345-353+405 |
分類號 | 418.31 |
關鍵詞 | 鈣; 上皮細胞; 肺癌; 白蔾蘆醇; Calcium; Epithelium; Lung cancer; Resveratrol; |
語 文 | 英文(English) |
中文摘要 | 目的:研究為探討白蔾蘆醇對人類肺腺癌細胞(A549)及正常豬氣管上皮細胞之作用,並且比較白蔾蘆醇對個別細胞之慣性反應。材料與方法:錐藍排除試驗和MTT試驗則檢測細胞的存活率及生長。細胞內鈣離子濃度利用螢光影像測量之。細胞凋亡蛋白的排除利利用西方點墨法分析。結果:顯示在濃度和時間依賴性下,白蔾蘆醇不僅引起細胞死亡而且抑制細胞生長。更重要地,白蔾蘆醇對於迷你豬氣管上皮細胞之慣性弱於人類肺腺癌細胞,表示肺腺癌細胞對白蔾蘆醇較具敏感性。更甚者白蔾蘆醇引起細胞死亡可能與細胞凋亡訊息相關,因白蔾蘆醇可增加細胞內鈣離子濃度及提升p53和Bax蛋白的表達。結論:白蔾蘆醇未來可能被應用成為治療及預防人類肺癌之新藥,因為正常呼吸道上皮細胞比肺腺癌細胞對於白蔾蘆醇之細胞毒性較具抵抗力。 |
英文摘要 | Objective: This study investigated the effects of resveratorl on cell growth in human lung adenocarinoma A549 cells and normal swine traheal epithelial cells (STECs), and compared their cytotoxic responses. Materials and Methods: Cell viability and cell growth were measured by trypan blue dye exclusion and MTT reduction assay. The intracellular C□ levels were determined by fluorescent imaging. Protein expression in apoptotic signaling was measured by Western blotting. Results: Data showed that resveratrol not only induced cell death but also inhibited cell growth in a concentration- and time-dependent manner. Importantly, resveratrol was less toxic to STECs than to lung adenocarcinoma, indicating that adenocarcinoma is largely sensitive to resveratrol. Furhtermore, cell death was consistent with the apoptotic signals triggered by resveratorl, with an increase in cytosolic C□ levels and a market increase in the expression of p53 and Bax proteins. Conclusion: Resveratrol may have potential for prevenetion and treatment of lung adenocarcinoma; normal airway epithelia were more resistance than lung adenocarcionoma to the cytotoxicity of resvertrol. |
本系統中英文摘要資訊取自各篇刊載內容。