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相關文獻
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頁籤選單縮合
題 名 | 水稻直鏈性澱粉突變體快速篩選法=A Fast Method for Screening Amylose Mutant Rice |
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作 者 | 鄭統隆; 林素月; 曾東海; 王強生; | 書刊名 | 臺灣農業研究 |
卷 期 | 54:2 民94.06 |
頁 次 | 頁103-112 |
分類號 | 434.113 |
關鍵詞 | 水稻; 直鏈性澱粉; 突變體; Rice; Amylose; Mutant; |
語 文 | 中文(Chinese) |
中文摘要 | 本試驗使用台農67號(TNG67)經疊氮化鈉誘變所得的低(7%)、高(30%)直鏈性澱粉含量的突變體,及直鏈性澱粉含量非純系之突變體SA0404為材料,並以中直鏈性澱粉含量(20%)之誘變親台農67號為對照品種,建立單粒米及半粒米之直鏈性澱粉測定方法,此方法節省人力。利用此方法不但可加速將突變體SA0404純化為直鏈性澱粉純系,且可應用於一穗內各穀粒間直鏈性澱粉含量變化之探討。本研究也確立同一米粒之頂端與基部之直鏈性澱粉含量並無明顯差異,不含胚之頂端可以代替開發為半粒米測定之代表,並建立半粒米之播種程序,更有助於追蹤並加速突變體之純化與篩選,及作為直鏈性澱粉特性之世代追蹤及加速品系選拔純化之相關研究用。 |
英文摘要 | Rice grains with various amylose contents from low(7%)、high(30%)and varied (non-purified) amylose mutants, as well as from their original variety, TNG67(20%), were applied in this study. A method with less labor cost for amylose analysis on a single grain and half grain is developed in this study. The distribution of amylose in grains of all spikelets on a panicle could be detected and planted to the next generation for further analysis, and a non-purified segregating mutant, SA0404, was quickly screened and purified by this method. Besides, our investigations found that there is no difference in amylose content between distal and proximal part of a single rice grain. In this report, a fast and reliable method for analyzing amylose content and plant growing system on a single rice grain was developed that will benefit to improve rice quality related amylose content. |
本系統中英文摘要資訊取自各篇刊載內容。