頁籤選單縮合
| 題 名 | Electroosmotic Mixing Induced by Non-uniform Zeta Potential and Application for DNA Microarray in Microfluidic Channel |
|---|---|
| 作 者 | Wei, Cheng-wey; Young, Tai-horng; Cheng, Ji-yen; | 書刊名 | 醫學工程 |
| 卷 期 | 17:6 民94.12 |
| 頁 次 | 頁281-283 |
| 分類號 | 410.1644 |
| 關鍵詞 | Microarray; Microchannel; PMMA; Electroosmotic mixing; Non-uniform zeta potential; |
| 語 文 | 英文(English) |
| 英文摘要 | In this study, we describe a modification of the conventional microarray format. 20-mer oligonucleotide probes and singly labeled 20-mer targets, representative of the T-cell acute lymphocytic leukemia 1 (TAL1) gene, has been used to elucidate the performance of this hybridization approach. DNA microarray is integrated with microfluidic channel on a poly(methyl methacrylate) (PMMA) to generate non-uniform zeta potential inside the channel. A microtrench is designed and fabricated on a PMMA chip using a widely available CO2 laser scriber. The electroosmotic mixing effect induced by the non-uniform zeta potential is utilized to enhance the DNA-DNA hybridization. The flow field in microfluidic channel is measured by particle image velocimetry (PIV). The enhanced signal to noise (S/B) ratio and reduced hybridization time is observed when the electroosmotic mixing is applied in the DNA-DNA hybridization. |
本系統中英文摘要資訊取自各篇刊載內容。