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頁籤選單縮合
題名 | 免疫去勢用抗原之生產及接種公土雞之免疫反應=Production of Immunocastration Antigen and the Immune Response of Taiwan Native Roosters after Vaccination |
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作者 | 蕭振文; 劉瑞珍; 翁佳君; 蔡麗卿; 陳立人; 黃祥吉; 楊惠郎; 戴謙; | 書刊名 | 畜產研究 |
卷期 | 38:3 民94.09 |
頁次 | 頁217-226 |
分類號 | 437.71 |
關鍵詞 | 免疫去勢; 激性腺素釋放素; 疫苗; 綠膿桿菌外毒素; Immunocastration; GnRH; Vaccine; Pseudomonas exotoxin; |
語文 | 中文(Chinese) |
中文摘要 | 本研究旨在研發免疫去勢抗原並接種公土雞之後評估及其對性腺發育之影響。試驗利用大腸桿菌(Escherichia coli, E. coli)表現之重組去毒性線膿桿菌外毒素(recombinant Pseudomonas exotoxin, rPE)估偽攜帶蛋白(carrier protein),與激性腺素釋放素(gonadotropin-releasing hormone, GnRH)化學連接後做為免疫原(GnRH-rPE),進行公土雞之免疫接種。試驗動物為畜試土雞公雞40隻,逢機分為4組,分別為正常對照組、磷酸緩衝液(phosphate-buffered saline, PBS)添加佐劑接種組、外科去勢組與GnRH-rPE接踵組,免疫原之接種採頸部皮下多點注射,於公土雞第3週、第6週及第16週齡接種3次抗原。結果顯示,接種GnRH-rPE之公雞能誘發抗-PE與抗-GnRH的抗體,然而公雞之外觀、雞冠發育、生精作用及睪丸重等均與正常之對照組與PBS接種組公雞無差異。由此結果顯示接種GnRH-rPE可誘發抗體產生,而的價卻未能有效拮抗GnRH功能而抑制公雞之性腺發育與生精作用。 |
英文摘要 | The purpose of this study was to develop an immunocastration antigen and to use it as vaccine to immunizing Taiwan native roosters. Gonadotropin-releasing hormone (GnRH) is a self-antigen and a small molecule that usually cannot elicit an immune response. To generate antibodies against GnRH, it is necessary to link GnRH to immuno-carriers. PE was produced by an opportunistic pathogen Pseudomonas aeruginosa. A truncated recombinant detoxicated PE (rPE) modified by gene deletion was used as an immuno-carrier which was conjugated chemically with GnRH. This GnRH-rPE conjugate was used to vaccinate roosters of native chickens. Forty roosters were randomly divided into four groups. They were castration, normal control, PBS control and GnRH-rPE vaccination groups, respectively. The roosters received immunization injections at 3, 6 and 16 weeks of age. Results showed that the roosters immunized with the GnRH-rPH were able to generate anti-GnRH and anti-PE antibodies. The GnRH-rPE vaccination roosters showed normal growth and development of reproductive organs. In conclusion, GnRH-rPE vaccination could induce antibody production of native roosters but the immune response against GnRH was not strong enough to inhibit the development of male reproductive organs. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。