頁籤選單縮合
題 名 | Micro Capillary Electrophoresis Chips with Sample Pre-Concentration Devices Utilizing Alternating Current (AC) Electroosmosis Effect |
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作 者 | Hsiung, Suz-kai; Tseng, Hao-yu; Lee, Gwo-bin; | 書刊名 | 淡江理工學刊 |
卷 期 | 8:3 民94.09 |
頁 次 | 頁237-244 |
分類號 | 448.5 |
關鍵詞 | AC electroosmosis; DNA analysis; Micro capillary electrophoresis; Pre-concentration; |
語 文 | 英文(English) |
英文摘要 | The present paper reports a new micro capillary electrophoresis (CE) chip integrated with a sample pre-concentration device utilizing alternating current (AC) electro-osmosis effect. Micro capillary electrophoresis chip has proven to be a powerful tool for sample separation. Not only does it miniaturize the size of the analytical apparatus, but is also executes samples reagents. However, the minimum detectable concentration of the samples could still remain an issue. To increase the detectable limits, pre-concentration micro-devices prior to sample separation/detection are of crucial needs. In this study, we utilize a pair of electrodes to generate AC electroosmosis forces such that DNA samples could be focused in a concentration zone, and thus increasing the fluorescence signals. A combination of high-voltage power supplies, high-voltage relays and function generators is used to apply the AC electric signals on the pre-concentration electrodes. Sample plugs in the microhannel are thus concentrated in the pre-concentration zone. Concentrated samples are then injection into the subsequent separation channels. At last, a PMT (Photo-multiplier tube) module is used to detect the fluorescence signals enhanced by the AC electroosmosis effect. Three DNA samples, including λ-DNA (12 bps), øX-174 DNA maker (11 segments) and detection gene for Group A streptococcus (777 bps), have been tested. Successful concentration and separation of these samples have been experimentally verified. The developed micro CE devices with the pre-concentration devices could have significant potential for the analysis of the dilute and low concentration DNA samples. |
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