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題 名 | 虎杖大量繁殖及品質鑑定之研究=Studies on the Clonal Propagation and Evaluation of the Quality of Polygonum Cuspidatum SIEB. et ZUCC. |
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作 者 | 關甫伈; | 書刊名 | 中醫藥年報 |
卷 期 | 25:4 2007.10[民96.10] |
頁 次 | 頁361-382 |
專 輯 | 中醫藥品質管理相關研究 |
分類號 | 414.32 |
關鍵詞 | 虎杖; 植物組織培養; 大黃素; Polygonum cuspidatum; Plant tissue culture; Emodin; |
語 文 | 中文(Chinese) |
中文摘要 | 虎杖(Polygoni Cuspidati Rhizoma)為中國臨床上風濕痺痛之常用中藥,其所含化學成分有大黃素、大黃素甲醚等蒽醌類化合物及白藜蘆醇等活性成分,現代藥理學研究亦顯示具有顯著的鎮咳、降壓、抗菌、抗病毒、降血脂及抑制血小板凝集等作用,其甚具經濟價值。因此本計畫針對省產虎杖植物之保存與開發利用等相關研究之進行。 臺灣土地已過度開發,虎杖野生資源日益減少,且通生長於低海拔的根莖較生長於高海拔的根莖結實,在栽培時,宜選擇適當之土壤、氣候等條件適合場地繁殖。為了開發虎杖藥材的資源利用及品質穩定,利用植物組織培養具有育種及大量繁殖之功能,利用植物組織培養技術,可短時間內獲得大量繁殖植物的特性,取代傳統育種,提昇虎杖品質的良好方法,並快速生產虎杖之二次代謝產物。 外界所採集的虎杖植物,經由TLC及HPLC針對虎杖所含大黃素量多寡,篩選品質較佳的虎杖植物,進行本研究的計畫。虎杖芽體以含有1.5 mg/L BA及0.5 mg/L NAA之MS培養基培養60天後,可產生4.8個不定芽。若改以液體與固體交替培養方式,對所誘導二次不定芽,可獲得13個不定芽。將芽體以含5% sucrose之MS培養基培養60天後可得良好根系,以此方式可建立虎杖之快速繁殖途徑。虎杖由根部所誘導之癒合組織,經暗培養45天後,以含MS基本鹽類1 mg/L 2,4-D與0.2 mg/L kinetin組合對癒荷組織生長,可獲得最佳癒合組織平均鮮重為70 mg,有助於建立優良之懸浮細胞系,藉此提升二次代謝物之產量。 |
英文摘要 | Polygonum cuspidatum Sieb. et Zucc. (Huzhang in Chinese) is a kind of traditional Chinese medicinal herb commonly used for the treatment of rheumatic fever. The major components of P. cuspidatum including emodin, physcion and resveratrol etc. The roots of the P. cuspidatum are used as an antherosclerosis, and other medical ailments, such as coughs, asthma, hypertension, and cancer. So this project makes use of to wait for the related research to the conservancy and development that the province produces the plant of P. cuspidatum. For the sake of the using of resource and quality that develop the P. cuspidatum medicine material the stability, the function that make use of the plant propagation to has to teach to grow and breed in great quantities, makes use of the plant propagation technique, can a short time inside the characteristic that acquire to breed the plant in great quantities, replace the tradition to teach the kind, promote the good method of the P. cuspidatum quality, and the fast production P. cuspiidatum of metabolize the outcome two times. The P. cuspidatum plant through the TLC and the HPLC with the greatly better P. cuspidatum plant of the sieving quality, carry on the project of this research. A simple protocol for in vitro mass propagation of P. cuspidatum has been developed. Multiple shoot was multiplied by subculturing on MS medium supplemented with 0.5 mg/L NAA and 1.5 mg/L BA. The shoots were multiplied by change to replace to foster the way with the liquid and the solid. Shoots were rooted on MS basal medium with 5% sucrose supplemented with various auxins. Shoots rooted on growth regulator with medium were transferred to soil: vermiculite mixture and acclimatized in the growth chamber. The roots of P. cuspidatum were cultured on a medium containing MS medium with 1 mg/L 2,4-D and the 0.2 mgs/L kinetin of inducing callus. The best callus to match the organization equally fresh and heavy for 70 mg, contribute to the establishment good suspension cell department. |
本系統中英文摘要資訊取自各篇刊載內容。